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Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2

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Title: Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2
Authors: Yoshida, Takashi Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Otani, Kaori Browse this author
Inoue, Kana Browse this author
Nakane, Kazuyasu Browse this author
Nishimura, Hiroyuki Browse this author
Ibara, Asako Browse this author
Shimada, Ayumu Browse this author
Ogawa, Kosuke Browse this author
Nishida, Erika Browse this author
Sugaya, Tsutomu Browse this author →KAKEN DB
Sun, Ling Browse this author
Fugetsu, Bunshi Browse this author →KAKEN DB
Kawanami, Masamitsu Browse this author →KAKEN DB
Keywords: β-tricalcium phosphate (β-TCP)
co-poly lactic acid/glycolic acid (PLGA)
fibroblast growth factor-2 (FGF2)
bone tissue engineering
Issue Date: May-2015
Publisher: Wiley-Blackwell
Journal Title: Journal of Periodontal Research
Volume: 50
Issue: 2
Start Page: 265
End Page: 273
Publisher DOI: 10.1111/jre.1 2206
PMID: 24966062
Abstract: Background and objective: β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the implantation of the PLGA/β-TCP scaffold loaded with fibroblast growth factor-2 (FGF2) on bone augmentation. Material and methods: The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture, and a subcutaneous implant test. Subsequently, a bone forming test was performed using fifty two rats. The β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffolds loaded with FGF2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 days post-surgery. Results: SEM and TEM observations showed a thin PLGA layer on the β-TCP particles after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater than the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation. Conclusion: The PLGA coating improved the mechanical strength of β-TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β-TCP scaffolds in combination with FGF2 are bioeffective for bone augmentation.
Rights: This is the peer reviewed version of the following article: [Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2], which has been published in final form at [ 2206]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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