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Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes

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JBC 291 (12)p.6559-6568.pdf1.07 MBPDF見る/開く
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タイトル: Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes
著者: Kobayashi, Shintaro 著作を一覧する
Suzuki, Tadaki 著作を一覧する
Kawaguchi, Akira 著作を一覧する
Phongphaew, Wallaya 著作を一覧する
Yoshii, Kentaro 著作を一覧する
Iwano, Tomohiko 著作を一覧する
Harada, Akihiro 著作を一覧する
Kariwa, Hiroaki 著作を一覧する
Orba, Yasuko 著作を一覧する
Sawa, Hirofumi 著作を一覧する
キーワード: Rab
vesicles
transport
Flavivirus
infection
発行日: 2016年 3月18日
出版者: American Society for Biochemistry and Molecular Biology (ASBMB)
誌名: Journal of Biological Chemistry (JBC)
巻: 291
号: 12
開始ページ: 6559
終了ページ: 6568
出版社 DOI: 10.1074/jbc.M115.712760
抄録: West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
Rights: This research was originally published in Journal of Biological Chemistry. Shintaro Kobayashi, Tadaki Suzuki, Akira Kawaguchi, Wallaya Phongphaew, Kentaro Yoshii, Tomohiko Iwano, Akihiro Harada, Hiroaki Kariwa, Yasuko Orba and Hirofumi Sawa. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes. The Journal of Biological Chemistry. 2016; 291(12): 6559-6568. © the American Society for Biochemistry and Molecular Biology.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/62049
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 小林 進太郎

 

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