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Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes

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Title: Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes
Authors: Kobayashi, Shintaro Browse this author →KAKEN DB
Suzuki, Tadaki Browse this author →KAKEN DB
Kawaguchi, Akira Browse this author
Phongphaew, Wallaya Browse this author
Yoshii, Kentaro Browse this author →KAKEN DB
Iwano, Tomohiko Browse this author
Harada, Akihiro Browse this author →KAKEN DB
Kariwa, Hiroaki Browse this author →KAKEN DB
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: Rab
Issue Date: 18-Mar-2016
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 291
Issue: 12
Start Page: 6559
End Page: 6568
Publisher DOI: 10.1074/jbc.M115.712760
Abstract: West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
Rights: This research was originally published in Journal of Biological Chemistry. Shintaro Kobayashi, Tadaki Suzuki, Akira Kawaguchi, Wallaya Phongphaew, Kentaro Yoshii, Tomohiko Iwano, Akihiro Harada, Hiroaki Kariwa, Yasuko Orba and Hirofumi Sawa. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes. The Journal of Biological Chemistry. 2016; 291(12): 6559-6568. © the American Society for Biochemistry and Molecular Biology.
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 進太郎

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