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Volume 64 Number 2 >

Application of next-generation sequencing to investigation of norovirus diversity in shellfish collected from two coastal sites in Japan from 2013 to 2014

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.64.2.113

Title: Application of next-generation sequencing to investigation of norovirus diversity in shellfish collected from two coastal sites in Japan from 2013 to 2014
Authors: Imamura, Saiki Browse this author
Haruna, Mika Browse this author
Goshima, Tomoko Browse this author
Kanezashi, Hiromi Browse this author
Okada, Tsukasa Browse this author
Akimoto, Keiko Browse this author
Keywords: Crassostrea gigas
Mitilus galloprovincialis
Next-generation sequencing
noroviruses
Issue Date: May-2016
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 64
Issue: 2
Start Page: 113
End Page: 122
Abstract: A better understanding of the role played by shellfish regarding the manner of pathogen contamination, persistence, and selection may help considering epidemiology of noroviruses. Thus, norovirus genotype profiles in shellfish (Crassostrea gigas and Mitilus galloprovincialis) were investigated by using Next-generation sequencing (NGS) technology. In genogroup I (GI), 7 genotypes (abbreviated as GI.2 to GI.7, and GI.9) were detected from C. gigas, whereas 9 genotypes (GI.1 to GI.9) were detected from M. galloprovincialis. The genotype with the highest proportion found in both C. gigas and M. galloprovincialis was GI.4, and the second highest was GI.3. In genogroup II (GII), 17 genotypes (GII.1 to GII.9, GII.11 to GII.17, GII.21 and GI.22) were detected from C. gigas, whereas 16 genotypes (GII.1 to GII.8, GII.11 to GII.17, GII.21 and GI.22) were detected from M. galloprovincialis. The genotype with the highest proportion in both C. gigas and M. galloprovincialis was GII.4, the next highest differed between C. gigas and M. galloprovincialis. To our knowledge, this study may be the first trial to utilize the latest technology in this field, and reveal the diversity of norovirus genotypes present in shellfish.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/62217
Appears in Collections:Japanese Journal of Veterinary Research > Volume 64 Number 2

Submitter: 獣医学部図書室

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