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Volume 64 Number 2 >

Canine osteosarcoma cell lines contain stem-like cancer cells : biological and pharmacological characterization

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Title: Canine osteosarcoma cell lines contain stem-like cancer cells : biological and pharmacological characterization
Authors: Gatti, Monica Browse this author
Wurth, Roberto Browse this author
Vito, Guendalina Browse this author
Pattarozzi, Alessandra Browse this author
Campanella, Chiara Browse this author
Thellung, Stefano Browse this author
Maniscalco, Lorella Browse this author
Maria, Raffaella De Browse this author
Villa, Valentina Browse this author
Corsaro, Alessandro Browse this author
Nizzari, Mario Browse this author
Bajetto, Adriana Browse this author
Ratto, Alessandra Browse this author
Ferrari, Angelo Browse this author
Barbieri, Federica Browse this author
Florio, Tullio Browse this author
Keywords: Osteosarcoma
cancer stem cells
comparative oncology
Issue Date: May-2016
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 64
Issue: 2
Start Page: 101
End Page: 112
Abstract: Cancer stem cells (CSCs) represent a small subpopulation of cells responsible for tumor formation and progression, drug resistance, tumor recurrence and metastasization. CSCs have been identified in many human tumors including osteosarcoma (OSA). CSC distinctive properties are the expression of stem cell markers, sustained growth, self-renewal and tumorigenicity. Here we report the isolation of stem-like cells from two canine OSA cultures, characterized by self-renewal, evaluated by sphere formation ability, differential marker expression, and in vitro proliferation when cultured in a medium containing EGF and bFGF. Current therapies for OSA increased survival time, but prognosis remains poor, due to the development of drug resistance and metastases. Chemotherapy shrinks the tumor mass but CSCs remain unaffected, leading to tumor recurrence. Metformin, a drug for type 2 diabetes, has been shown to possess antitumor properties affecting CSC survival in different human and animal cancers. Here we show that metformin has a significant antiproliferative effect on canine OSA stem-like cells, validating this in vitro model for further pre-clinical drug evaluations. In conclusion, our results demonstrate the feasibility of obtaining CSC-enriched cultures from primary canine OSA cells as a promising model for biological and pharmacological studies of canine and human OSAs.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 64 Number 2

Submitter: 獣医学部図書室

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