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Metabolic Activation of Heterocyclic Amines and Expression of Xenobiotic-Metabolizing Enzymes in the Gastrointestinal Tract of Rats

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/62339

Title: Metabolic Activation of Heterocyclic Amines and Expression of Xenobiotic-Metabolizing Enzymes in the Gastrointestinal Tract of Rats
Authors: Darwish, Wageh S. Browse this author
Nakayama, Shouta M. M. Browse this author →KAKEN DB
Itotani, Yuumi Browse this author
Ohno, Marumi Browse this author
Ikenaka, Yoshinori Browse this author →KAKEN DB
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: Gastrointestinal Tract
Xenobiotic-Metabolizing Enzymes
Heterocyclic Amines
Issue Date: Jul-2015
Publisher: Wiley-Blackwell
Journal Title: Journal of Food Science
Volume: 80
Issue: 7
Start Page: T1627
End Page: T1632
Publisher DOI: 10.1111/1750-3841.12931
Abstract: Heterocyclic amines get entry into human body mainly through ingestion of pan-fried meats cooked at high temperatures. Exposure of the gastrointestinal tract (GIT) to ingested xenobiotics prior to delivery to the liver may lead to metabolic activation, which may explain the high incidence of GIT carcinogenesis. Therefore, this study investigated the mutagenic activation of 2 heterocyclic amines, 2-aminoanthracene (2-AA) and 3-amino-1-methyl-5H-prydo[4,3-b]indole (Trp-P-2), in the GIT of rats. In addition, the constitutive mRNA expression profiles of xenobiotic-metabolizing enzymes (XMEs) in the GIT of rats were examined. Metabolic activation of 2-AA was detected in all GIT tissues except the duodenum and rectum, and it was detected at high levels in the ileum and cecum. Furthermore, we revealed high metabolic activation of 2-AA and Trp-P-2 in the jejunum. The mRNA expression of phase I and II enzymes in rat GIT corresponded with their mutagenic activation ability. In conclusion, our results suggest that different expression levels of XME among GIT tissues may contribute to the tissue-specific differences in metabolic activation of xenobiotics such as heterocyclic amines in rats. Practical Application This study declares mutagenic activation of 2 heterocyclic amines namely 2-aminoanthracene (2-AA) and 3-amino-1-methyl-5H-prydo[4,3-b]indole (Trp-P-2), in the gastrointestinal tract (GIT) of rats. In addition, results obtained in this study suggest that GIT tissue-specific expression of xenobiotic metabolizing enzymes may contribute to the tissue-specific mutagenesis/carcinogenesis.
Rights: This is the peer reviewed version of the following article: Darwish WS, Nakayama SM, Itotani Y, Ohno M, Ikenaka Y, Ishizuka M. 2015. Metabolic activation of heterocyclic amines and expression of xenobiotic-metabolizing enzymes in the gastrointestinal tract of rats. J Food Sci 80:T1627-32, which has been published in final form at http://dx.doi.org/10.1111/1750-3841.12931. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Type: article (author version)
URI: http://hdl.handle.net/2115/62339
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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