| Title: | Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent : a pilot study |
| Authors: | Mizushima, Takeshi Browse this author |
| Ohnishi, Shunsuke Browse this author →KAKEN DB |
| Shimizu, Yuichi Browse this author →KAKEN DB |
| Hatanaka, Yutaka Browse this author →KAKEN DB |
| Hatanaka, Kanako C. Browse this author |
| Hosono, Hidetaka Browse this author |
| Kubota, Yoshimasa Browse this author |
| Natsuizaka, Mitsuteru Browse this author |
| Kamiya, Mako Browse this author →KAKEN DB |
| Ono, Shouko Browse this author |
| Homma, Akihiro Browse this author →KAKEN DB |
| Kato, Mototsugu Browse this author →KAKEN DB |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Urano, Yasuteru Browse this author |
| Keywords: | Fluorescent imaging |
| γ-Glutamyltranspeptidase |
| Head and neck squamous cell carcinoma |
| Issue Date: | 7-Jul-2016 |
| Publisher: | BioMed Central |
| Journal Title: | BMC Cancer |
| Volume: | 16 |
| Start Page: | 411 |
| Publisher DOI: | 10.1186/s12885-016-2421-z |
| Abstract: | Background: Detecting superficial head and neck squamous cell carcinoma (HNSCC) by endoscopy is challenging because of limited morphological hallmarks, and iodine cannot be applied to head and neck lesions due to severe mucosal irritation. γ-glutamyltranspeptidase (GGT), a cell surface enzyme, is overexpressed in several cancers, and it has been reported that γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a fluorescent targeting agent which can be enzymatically activated and becomes fluorescent after cleavage of a GGT-specific sequence, can be activated within a few minutes after application to animal models. We investigated whether early HNSCC can be detected by applying gGlu-HMRG to clinical samples. Methods: gGlu-HMRG was applied to four HNSCC cell lines, and fluorescence was observed by fluorescence microscopy and flow cytometry. Immunohistological examination was performed in three recent cases of endoscopic submucosal dissection (ESD) to investigate GGT expression. Fluorescence imaging with gGlu-HMRG in eight clinical samples resected by ESD or surgery was performed, and fluorescence intensity of tumor and normal mucosa regions of interest (ROI) was prospectively measured. Results: All four gGlu-HMRG-applied cell lines emitted green fluorescence. Immunohistological examination demonstrated that GGT was highly expressed in HNSCC of the recent three ESD cases but barely in the normal mucosa. Fluorescence imaging showed that iodine-voiding lesions became fluorescent within a few minutes after application of gGlu-HMRG in all eight resected tumors. Tumor ROI fluorescence intensity was significantly higher than in the normal mucosa five minutes after gGlu-HMRG application. Conclusions: Fluorescence imaging with gGlu-HMRG would be useful for early detection of HNSCC. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/62385 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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