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NETosis markers: Quest for specific, objective, and quantitative markers

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Title: NETosis markers: Quest for specific, objective, and quantitative markers
Authors: Masuda, Sakiko Browse this author
Nakazawa, Daigo Browse this author
Shida, Haruki Browse this author
Miyoshi, Arina Browse this author
Kusunoki, Yoshihiro Browse this author
Tomaru, Utano Browse this author →KAKEN DB
Ishizu, Akihiro Browse this author →KAKEN DB
Keywords: Neutrophil extracellular trap
Peptidylarginine deiminase 4
Citrullinated histone; MPO-DNA complex
Flow cytometry
Issue Date: 1-Aug-2016
Publisher: Elsevier
Journal Title: Clinica Chimica Acta
Volume: 459
Start Page: 89
End Page: 93
Publisher DOI: 10.1016/j.cca.2016.05.029
PMID: 27259468
Abstract: More than 10 years have passed since the discovery of neutrophil extracellular traps (NETs) in 2004. NETs are extracellular web-like DNA decorated with antimicrobial proteins, which are released from activated neutrophils. The state of neutrophils with NET formation is called NETosis. It has been realized that NETosis includes suicidal NETosis and vital NETosis. The former state means cell death of neutrophils, whereas the latter state preserves living neutrophilic functions. Although both suicidal and vital NETosis play essential roles in elimination of microorganisms, excessive formation of NETs, especially the ones derived from suicidal NETosis, can harm the hosts. Therefore, the discovery of NETosis markers and development of evaluation methods are important. In this review, we compare the methods for evaluating NETosis, including immunocytological and immunohistological detection of co-localized neutrophil-derived proteins and extracellular DNA, and citrullinated histones, detection of NET remnants in fluid samples, and flow cytometric detection of cell-appendant NET components, with focus on the specificity, objectivity, and quantitativity. Since the gold standard marker of NETosis or method of NET detection has not been established yet, researchers should choose the most appropriate marker or method in each situation based on the knowledge of the respective virtues and faults.
Rights: ©2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Type: article
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石津 明洋

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