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In vivo imaging of clock gene expression in multiple tissues of freely moving mice

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Title: In vivo imaging of clock gene expression in multiple tissues of freely moving mice
Authors: Hamada, Toshiyuki Browse this author →KAKEN DB
Sutherland, Kenneth Browse this author →KAKEN DB
Ishikawa, Masayori Browse this author →KAKEN DB
Miyamoto, Naoki Browse this author →KAKEN DB
Honma, Sato Browse this author →KAKEN DB
Shirato, Hiroki Browse this author →KAKEN DB
Honma, Ken-ichi Browse this author →KAKEN DB
Issue Date: 10-Jun-2016
Publisher: Nature
Journal Title: Nature communications
Volume: 7
Start Page: 11705
Publisher DOI: 10.1038/ncomms11705
PMID: 27285820
Abstract: Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.
Type: article
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石川 正純

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