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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
In vivo imaging of clock gene expression in multiple tissues of freely moving mice
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | In vivo imaging of clock gene expression in multiple tissues of freely moving mice |
| Authors: | Hamada, Toshiyuki Browse this author →KAKEN DB | | Sutherland, Kenneth Browse this author →KAKEN DB | | Ishikawa, Masayori Browse this author →KAKEN DB | | Miyamoto, Naoki Browse this author →KAKEN DB | | Honma, Sato Browse this author →KAKEN DB | | Shirato, Hiroki Browse this author →KAKEN DB | | Honma, Ken-ichi Browse this author →KAKEN DB |
| Issue Date: | 10-Jun-2016 |
| Publisher: | Nature |
| Journal Title: | Nature communications |
| Volume: | 7 |
| Start Page: | 11705 |
| Publisher DOI: | 10.1038/ncomms11705 |
| PMID: | 27285820 |
| Abstract: | Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/62597 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石川 正純
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