| Title: | The RNA Sensor RIG-I Dually Functions as an Innate Sensor and Direct Antiviral Factor for Hepatitis B Virus |
| Other Titles: | RIG-I functions as a dual effector against HBV |
| Authors: | Sato, Seiichi Browse this author |
| Li, Kai Browse this author |
| Kameyama, Takeshi Browse this author →KAKEN DB |
| Hayashi, Takaya Browse this author |
| Ishida, Yuji Browse this author |
| Murakami, Shuko Browse this author →KAKEN DB |
| Watanabe, Tsunamasa Browse this author →KAKEN DB |
| Iijima, Sayuki Browse this author →KAKEN DB |
| Sakurai, Yu Browse this author |
| Watashi, Koichi Browse this author →KAKEN DB |
| Tsutsumi, Susumu Browse this author |
| Sato, Yusuke Browse this author →KAKEN DB |
| Akita, Hidetaka Browse this author →KAKEN DB |
| Wakita, Takaji Browse this author →KAKEN DB |
| Rice, Charles M. Browse this author |
| Harashima, Hideyoshi Browse this author →KAKEN DB |
| Kohara, Michinori Browse this author →KAKEN DB |
| Tanaka, Yasuhito Browse this author →KAKEN DB |
| Takaoka, Akinori Browse this author →KAKEN DB |
| Issue Date: | 20-Jan-2015 |
| Publisher: | Cell Press |
| Journal Title: | Immunity |
| Volume: | 42 |
| Issue: | 1 |
| Start Page: | 123 |
| End Page: | 132 |
| Publisher DOI: | 10.1016/j.immuni.2014.12.016 |
| PMID: | 25557055 |
| Abstract: | Host innate recognition triggers key immune responses for viral elimination. The sensing mechanism of hepatitis B virus (HBV), a DNA virus, and the subsequent downstream signaling events remain to be fully clarified. Here we found that type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. In addition, RIG-I could also counteract the interaction of HBV polymerase (P protein) with the 5'-ε region in an RNA-binding dependent manner, which consistently suppressed viral replication. Liposome-mediated delivery and vector-based expression of this ε region-derived RNA in liver abolished the HBV replication in human hepatocyte-chimeric mice. These findings identify an innate recognition mechanism by which RIG-I dually functions as an HBV sensor activating innate signaling and to counteract viral polymerase in human hepatocytes. |
| Description: | Supplemental materials are available on the publisher's website. |
| Rights: | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/62620 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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