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Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/62623

Title: Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells
Authors: Oasa, Sho Browse this author
Sasaki, Akira Browse this author
Yamamoto, Johtaro Browse this author
Mikuni, Shintaro Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Keywords: Dissociation constant
Fluorescence correlation spectroscopy
Glucocorticoid receptor
Homodimerization
Microwell
Single cell
Issue Date: 4-Aug-2015
Publisher: WILEY
Journal Title: FEBS Letters
Volume: 589
Issue: 17
Start Page: 2171
End Page: 2178
Publisher DOI: 10.1016/j.febslet.2015.07.003
PMID: 26183204
Abstract: Glucocorticoid receptor alpha (GR) binds to the promoter regions of target genes as a homodimer and activates its transcriptional process. Though the homodimerization is thought to be the initial and essential process, the dissociation constant for homodimerization of GR remains controversial. To quantify homodimerization of (enhanced green fluorescence protein) EGFP-(glucocorticoid receptor) GR, the particle brightness in lysates from single cell was estimated for the fraction of homodimeric EGFP-GR using fluorescence correlation spectroscopy and microwells. Fitting the data with a bimolecular reaction model, the dissociation constant was determined. Moreover slow-diffusion complex was observed. These results suggest that EGFP-GR forms not only a monomer-dimer equivalent state but also a large-molecular-weight complex.
Rights: This is the peer reviewed version of the following article: FEBS Letters Vol. 589 (17) p.2171-2178 2015, which has been published in final form at 10.1016/j.febslet.2015.07.003. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/62623
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金城 政孝

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