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The Sam68 nuclear body is composed of two RNase-sensitive substructures joined by the adaptor HNRNPL

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Title: The Sam68 nuclear body is composed of two RNase-sensitive substructures joined by the adaptor HNRNPL
Authors: Mannen, Taro Browse this author
Yamashita, Seisuke Browse this author
Tomita, Kozo Browse this author
Goshima, Naoki Browse this author →KAKEN DB
Hirose, Tetsuro Browse this author →KAKEN DB
Issue Date: 4-Jul-2016
Publisher: Rockefeller University Press
Journal Title: Journal of cell biology
Volume: 214
Issue: 1
Start Page: 45
End Page: 59
Publisher DOI: 10.1083/jcb.201601024
Abstract: The mammalian cell nucleus contains membraneless suborganelles referred to as nuclear bodies (NBs). Some NBs are formed with an architectural RNA (arcRNA) as the structural core. Here, we searched for new NBs that are built on unidentified arcRNAs by screening for ribonuclease (RNase)-sensitive NBs using 32,651 fluorescently tagged human cDNA clones. We identified 32 tagged proteins that required RNA for their localization in distinct nuclear foci. Among them, seven RNA-binding proteins commonly localized in the Sam68 nuclear body (SNB), which was disrupted by RNase treatment. Knockdown of each SNB protein revealed that SNBs are composed of two distinct RNase-sensitive substructures. One substructure is present as a distinct NB, termed the DBC1 body, in certain conditions, and the more dynamic substructure including Sam68 joins to form the intact SNB. HNRNPL acts as the adaptor to combine the two substructures and form the intact SNB through the interaction of two sets of RNA recognition motifs with the putative arcRNAs in the respective substructures.
Rights: ©Mannen et al., 2016. Originally published in the journal of cell biology. doi:10.1083/jcb.201601024
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 廣瀬 哲郎

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