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Volume 64 Number 3 >

Alleviative effect of some phytochemicals on cyadox-induced oxidative damage in rabbit erythrocytes

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Title: Alleviative effect of some phytochemicals on cyadox-induced oxidative damage in rabbit erythrocytes
Authors: Farag, Mayada R. Browse this author
Alagawany, Mahmoud Browse this author
Abd El-Hack, Mohamed E. Browse this author
Tufarelli, Vincenzo Browse this author
Keywords: Cyadox
oxidative stress
response curve
Issue Date: Aug-2016
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 64
Issue: 3
Start Page: 171
End Page: 182
Abstract: This study was carried out to evaluate the effects of different concentrations of cyadox (CYA), cinnamaldehyde (CIN) and resveratrol (RES) on rabbit isolated erythrocytes in two experiments. Experiment 1 evaluated the effects of different concentrations (2.5, 5, 10, 20, 40, 60, 80 and 100) μg/ml of CYA and μM of both CIN and RES separately on isolated erythrocytes in order to make the concentration response curve. Results indicated that CYA caused a significant depletion of reduced glutathione (GSH) content compared to control at 40 μg/ml and the depletion increased by increasing the concentration. On the other hand, both RES and CIN showed a highly significant elevation of GSH content at 40 μM being more effective by increasing concentrations. Experiment 2 investigated the potential benefits of using CIN and RES separately or in combination on CYA induced alterations in isolated rabbit erythrocytes (the used concentrations were based on the results of experiment 1). Results revealed that exposure to CYA caused a significant decrease in superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) and total protein (TP) contents, CYA also elevated extracellular hemoglobin (Hb) and adenosine triphosphate (ATP), increased the malonaldehyde (MDA) and protein carbonyl (PrC) contents with increasing caspase3 and caspase8 activities suggesting CYA pro-oxidant effect. Both CIN and RES were able to inverse these hazardous effects of CYA. However, CIN was more effective than RES, and their combination showed a positive synergistic effect in protecting cells against oxidative injury caused by CYA.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 64 Number 3

Submitter: 獣医学部図書室

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