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Perilipin overexpression in mice protects against diet-induced obesity

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Title: Perilipin overexpression in mice protects against diet-induced obesity
Authors: Miyoshi, Hideaki Browse this author →KAKEN DB
Souza, Sandra C. Browse this author
Endo, Mikiko Browse this author
Sawada, Takashi Browse this author
Perfield, James W. II Browse this author
Shimizu, Chikara Browse this author →KAKEN DB
Stancheva, Zlatina Browse this author
Nagai, So Browse this author →KAKEN DB
Strissel, Katherine J. Browse this author
Yoshioka, Narihito Browse this author →KAKEN DB
Obin, Martin S. Browse this author
Koike, Takao Browse this author →KAKEN DB
Greenberg, Andrew S. Browse this author
Keywords: adipocyte
transgenic mice
lipid oxidation
glucose tolerance
insulin resistance
Issue Date: May-2010
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: Journal of Lipid Research
Volume: 51
Issue: 5
Start Page: 975
End Page: 982
Publisher DOI: 10.1194/jlr.M002352
Abstract: Perilipin A is the most abundant phosphoprotein on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Perilipin null mice exhibit diminished adipose tissue, elevated basal lipolysis, reduced catecholamine-stimulated lipolysis, and increased insulin resistance. To understand the physiological consequences of increased perilipin expression in vivo, we generated transgenic mice that overexpressed either human or mouse perilipin using the adipocyte-specific aP2 promoter/enhancer. Phenotypes of female transgenic and wild-type mice were characterized on chow and high-fat diets (HFDs). When challenged with an HFD, transgenic mice exhibited lower body weight, fat mass, and adipocyte size than wild-type mice. Expression of oxidative genes was increased and lipogenic genes decreased in brown adipose tissue of transgenic mice. Basal and catecholamine-stimulated lipolysis was decreased and glucose tolerance significantly improved in transgenic mice fed a HFD. Perilipin overexpression in adipose tissue protects against HFD-induced adipocyte hypertrophy, obesity, and glucose intolerance. Alterations in brown adipose tissue metabolism may mediate the effects of perilipin overexpression on body fat, although the mechanisms by which perilipin overexpression alters brown adipose tissue metabolism remain to be determined. Our findings demonstrate a novel role for perilipin expression in adipose tissue metabolism and regulation of obesity and its metabolic complications.
Rights: This research was originally published in Journal of Lipid Research. Miyoshi,H., S. C. Souza, M. Endo, T. Sawada, J. W. Perfi eld II, C. Shimizu, Z. Stancheva, S. Nagai, K. J. Strissel, N. Yoshioka, M. S. Obin, T. Koike, and A. S. Greenberg. Perilipin overexpression in mice protects against diet-induced obesity. J. Lipid Res. 2010. 51: 975–982. © the American Society for Biochemistry and Molecular Biology.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小池 隆夫

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