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Meiotic chromosome configurations in triploid progeny from reciprocal crosses between wild-type diploid and natural tetraploid loach Misgurnus anguillicaudatus in China

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Title: Meiotic chromosome configurations in triploid progeny from reciprocal crosses between wild-type diploid and natural tetraploid loach Misgurnus anguillicaudatus in China
Authors: Li, Ya-Juan Browse this author
Gao, Yang-Chun Browse this author
Zhou, He Browse this author
Ma, Hai-Yan Browse this author
Li, Jia-Qi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: bivalent
gamete
hybrid
polyploid
trivalent
univalent
Issue Date: Oct-2015
Publisher: springer
Journal Title: Genetica
Volume: 143
Issue: 5
Start Page: 555
End Page: 562
Publisher DOI: 10.1007/s10709-015-9853-2
Abstract: Here, we showed meiotic chromosome configurations prepared from oocyte germinal vesicles and spermatocytes of triploid loaches produced from reciprocal crosses between wild-type diploids (2n = 50) obtained from Dalian, Liaoning Province, China and natural tetraploids (4n = 100) from Chibi, Hubei Province, China. Major meiotic cells in triploids comprised 25 bivalents and 25 univalents, but cells with one to five trivalents were also observed. When three homologous chromosomes bearing nucleolar organizing regions (NOR) were identified with the detection of signals or positive sites by silver staining, chromomycin A3 staining and fluorescence in situ hybridization with a 5.8S + 28S rDNA probe, two third of selected triploid cells gave a configuration including one bivalent with two NORs (association of two homologous chromosomes) and one univalent with one NOR. However, other triploid cells showed three univalent each of which had one NOR, suggesting a failure of synapsis between homologous chromosomes. These results suggested that triploid female and male should produce aneuploid gametes with the theoretical mode at 1.5n (37 or 38 chromosomes).
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/62882
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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