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Decreased secretion of Paneth cell α-defensins in graft-versus-host disease

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Title: Decreased secretion of Paneth cell α-defensins in graft-versus-host disease
Other Titles: Fecal α-defensins in GVHD
Authors: Eriguchi, Y. Browse this author
Nakamura, K. Browse this author
Hashimoto, D. Browse this author
Shimoda, S. Browse this author
Shimono, N. Browse this author
Akashi, K. Browse this author
Ayabe, T. Browse this author
Teshima, T.8 Browse this author →KAKEN DB
Authors(alt): Teshima, Takanori8
Keywords: graft-versus-host disease
hematopoietic stem cell transplantation
α-defensins
cryptdin
mouse model
Paneth cells
fecal testing
microbiota
Issue Date: Oct-2015
Publisher: Wiley-Blackwell
Journal Title: Transplant infectious disease
Volume: 17
Issue: 5
Start Page: 702
End Page: 706
Publisher DOI: 10.1111/tid.12423
PMID: 26198302
Abstract: Background: Intestinal microbial ecology is actively regulated by Paneth cell-derived antimicrobial peptides, α-defensins. Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (SCT). We previously demonstrated that Paneth cells are targeted by GVHD, and their expression of antimicrobial peptide α-defensins is impaired, leading to a loss of physiological diversity among the microflora and development of bloodstream infection. Herein, we evaluated whether fecal levels of α-defensins could be surrogate marker of intestinal dysbiosis. Methods: We directly measured α-defensin cryptdin-1 (Crp1) in fecal pellets of mice with GVHD by using a novel enzyme-linked immunosorbent assay. Results: Fecal levels of Crp1 were significantly decreased in mice with GVHD but unchanged in mice without GVHD after SCT. These were correlated with intestinal flora diversity. Conclusion: We demonstrate a link between reduced secretion of Paneth cell α-defensins and dysbiosis of intestinal flora in GVHD. Fecal levels of α-defensins could be surrogate markers for intestinal microbial homeostasis.
Rights: This is the peer reviewed version of the following article: Y. Eriguchi, K. Nakamura, D. Hashimoto, S. Shimoda, N. Shimono, K. Akashi, T. Ayabe, T. Teshima. Decreased secretion of Paneth cell α-defensins in graft-versus-host disease. Transpl Infect Dis 2015: 17: 702–706., which has been published in final form at http://dx.doi.org/10.1111/tid.12423. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/62922
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 豊嶋 崇徳

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