HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
獣医学研究科  >
雑誌発表論文等  >

Bidirectional effects of hydrogen sulfide via ATP-sensitive K+ channels and transient receptor potential A1 channels in RIN14B cells

この資料はクリエイティブ・コモンズ・ライセンスの下で公開されています。

フルテキスト
Eur.J.Pharmacol.764p463-470.pdf508 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/62958

タイトル: Bidirectional effects of hydrogen sulfide via ATP-sensitive K+ channels and transient receptor potential A1 channels in RIN14B cells
著者: Ujike, Ayako 著作を一覧する
Otsuguro, Ken-ichi 著作を一覧する
Miyamoto, Ryo 著作を一覧する
Yamaguchi, Soichiro 著作を一覧する
Ito, Shigeo 著作を一覧する
キーワード: Hydrogen sulfide
Ca2+ signal
TRPA1
K-ATP channels
RIN14B cells
発行日: 2015年10月 5日
出版者: Elsevier
誌名: European Journal of Pharmacology
巻: 764
開始ページ: 463
終了ページ: 470
出版社 DOI: 10.1016/j.ejphar.2015.07.029
抄録: Hydrogen sulfide (H2S) reportedly acts as a gasotransmitter because it mediates various cellular responses through several ion channels including ATP-sensitive K+ (K-ATP) channels and transient receptor potential (TRP) A1 channels. H2S can activate both K-ATP, and TRPA1 channels at a similar concentration range. In a single cell expressing both channels, however, it remains unknown what happens when both channels are simultaneously activated by H2S. In this study, we examined the effects of H2S on RIN14B cells that express both K-ATP and TRPA1 channels. RIN14B cells showed several intracellular Ca2+ concentration ([Ca2+](i)) responses to NaHS (300 mu M), an H2S donor, i.e., inhibition of spontaneous Ca2+ oscillations (37%), inhibition followed by [Ca2+](i) increase (24%), and a rapid increase in [Ca2+](i) (25%). K-ATP channel blockers, glibenclamide or tolbutamide, abolished any inhibitory effects of NaHS and enhanced NaHS-mediated [Ca2+](i) increases, which were inhibited by extracellular Ca2+ removal, HC030031 (a TRPA1 antagonist), and disulfide bond-reducing agents. NaHS induced 5-hydroxytryptamine (5-HT) release from RIN14B cells, which was also inhibited by TRPA1 antagonists. These results indicate that H2S has both inhibitory and excitatory effects by opening K-ATP and TRPA1 channels, respectively, in RIN14B cells, suggesting potential bidirectional modulation of secretory functions. (C) 2015 Elsevier B.V. All rights reserved.
Rights: ©2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/62958
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 乙黒 兼一

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学