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Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

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Title: Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)
Authors: OKABE, Hiroaki Browse this author
HUI, Shu-Ping Browse this author
FUDA, Hirotoshi Browse this author
FURUKAWA, Takayuki Browse this author
TAKEDA, Seiji Browse this author
SHRESTHA, Rojeet Browse this author
MIURA, Yusuke Browse this author
WATANABE, Mitsugu Browse this author
CHIBA, Hitoshi Browse this author →KAKEN DB
Keywords: Amphipathicity
Pacific oyster
polar end-capped ODS
Issue Date: Dec-2015
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 31
Issue: 12
Start Page: 1341
End Page: 1344
Publisher DOI: 10.2116/analsci.31.1341
PMID: 26656828
Abstract: A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.
Type: article
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古川 貴之

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