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Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

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タイトル: Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)
著者: OKABE, Hiroaki 著作を一覧する
HUI, Shu-Ping 著作を一覧する
FUDA, Hirotoshi 著作を一覧する
FURUKAWA, Takayuki 著作を一覧する
TAKEDA, Seiji 著作を一覧する
SHRESTHA, Rojeet 著作を一覧する
MIURA, Yusuke 著作を一覧する
WATANABE, Mitsugu 著作を一覧する
CHIBA, Hitoshi 著作を一覧する
キーワード: Amphipathicity
polyphenol
LC-MS/MS
antioxidant
Pacific oyster
polar end-capped ODS
発行日: 2015年12月
出版者: 日本分析化学会(Japan Society for Analytical Chemistry)
誌名: Analytical Sciences
巻: 31
号: 12
開始ページ: 1341
終了ページ: 1344
出版社 DOI: 10.2116/analsci.31.1341
抄録: A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.
資料タイプ: article
URI: http://hdl.handle.net/2115/62970
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 古川 貴之

 

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