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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)
| Title: | Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas) |
| Authors: | OKABE, Hiroaki Browse this author | | HUI, Shu-Ping Browse this author | | FUDA, Hirotoshi Browse this author | | FURUKAWA, Takayuki Browse this author | | TAKEDA, Seiji Browse this author | | SHRESTHA, Rojeet Browse this author | | MIURA, Yusuke Browse this author | | WATANABE, Mitsugu Browse this author | | CHIBA, Hitoshi Browse this author →KAKEN DB |
| Keywords: | Amphipathicity | | polyphenol | | LC-MS/MS | | antioxidant | | Pacific oyster | | polar end-capped ODS |
| Issue Date: | Dec-2015 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 31 |
| Issue: | 12 |
| Start Page: | 1341 |
| End Page: | 1344 |
| Publisher DOI: | 10.2116/analsci.31.1341 |
| PMID: | 26656828 |
| Abstract: | A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/62970 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 古川 貴之
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