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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes
| Title: | Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes |
| Authors: | Ma, Yi-Shing Browse this author | | Yoshida, Shigeru Browse this author →KAKEN DB | | Kobayashi, Yu Browse this author | | Kawanishi, Noriaki Browse this author | | Furukawa, Takayuki Browse this author →KAKEN DB | | Fuda, Hirotoshi Browse this author | | Hui, Shu-Ping Browse this author →KAKEN DB | | Chiba, Hitoshi Browse this author →KAKEN DB |
| Keywords: | fatty acid uptake | | polyphenol | | mitochondrial respiration | | electrical stimulation | | C2C12 myotubes |
| Issue Date: | 2016 |
| Publisher: | Science & Education Publishing |
| Journal Title: | Journal of Food and Nutrition Research |
| Volume: | 4 |
| Issue: | 8 |
| Start Page: | 498 |
| End Page: | 507 |
| Publisher DOI: | 10.12691/jfnr-4-8-3 |
| Abstract: | Anti-oxidative effects of the Pacific oyster-derived phenolic antioxidant, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), has been documented in hepatocytes. Additionally, DHMBA-rich oyster extracts significantly attenuated obesity in a non-alcoholic steatohepatitis mouse model. Whether the administration of DHMBA might improve muscular mitochondrial function was investigated. The mouse C2C12-derived myotubes were loaded with oleic acid (400μM) and cultured for 24 hours in the presence of DHMBA (500μM) with or without electrical stimulation (ES), where ES was given as exercise mimic. The fatty acid uptake, lipid accumulation, and mitochondrial function were subsequently accessed. DHMBA and ES increased fatty acid uptake, TG contents, mitochondrial membrane potential, intracellular level of H2O2, and mitochondrial O2 consumption rate. Intracellular ATP content was significantly increased when both DHMBA and ES were loaded at the same time, suggesting their synergic action. Phosphorylated AMPKα, AMPKβ1, and acetyl-CoA carboxylase were increased by DHMBA, indicating a possible role for DHMBA for activation of metabolic adaptation system and consequent increase of fatty acid oxidation. In conclusion, DHMBA solely or in collaboration with exercise might possibly serve as a fitness food for obese persons by stimulating muscular fatty acid utilization and mitochondrial energy production. This assumption must be verified by animal experiment. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/62976 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 古川 貴之
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