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Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes

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Title: Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes
Authors: Ma, Yi-Shing Browse this author
Yoshida, Shigeru Browse this author →KAKEN DB
Kobayashi, Yu Browse this author
Kawanishi, Noriaki Browse this author
Furukawa, Takayuki Browse this author →KAKEN DB
Fuda, Hirotoshi Browse this author
Hui, Shu-Ping Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Keywords: fatty acid uptake
polyphenol
mitochondrial respiration
electrical stimulation
C2C12 myotubes
Issue Date: 2016
Publisher: Science & Education Publishing
Journal Title: Journal of Food and Nutrition Research
Volume: 4
Issue: 8
Start Page: 498
End Page: 507
Publisher DOI: 10.12691/jfnr-4-8-3
Abstract: Anti-oxidative effects of the Pacific oyster-derived phenolic antioxidant, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), has been documented in hepatocytes. Additionally, DHMBA-rich oyster extracts significantly attenuated obesity in a non-alcoholic steatohepatitis mouse model. Whether the administration of DHMBA might improve muscular mitochondrial function was investigated. The mouse C2C12-derived myotubes were loaded with oleic acid (400μM) and cultured for 24 hours in the presence of DHMBA (500μM) with or without electrical stimulation (ES), where ES was given as exercise mimic. The fatty acid uptake, lipid accumulation, and mitochondrial function were subsequently accessed. DHMBA and ES increased fatty acid uptake, TG contents, mitochondrial membrane potential, intracellular level of H2O2, and mitochondrial O2 consumption rate. Intracellular ATP content was significantly increased when both DHMBA and ES were loaded at the same time, suggesting their synergic action. Phosphorylated AMPKα, AMPKβ1, and acetyl-CoA carboxylase were increased by DHMBA, indicating a possible role for DHMBA for activation of metabolic adaptation system and consequent increase of fatty acid oxidation. In conclusion, DHMBA solely or in collaboration with exercise might possibly serve as a fitness food for obese persons by stimulating muscular fatty acid utilization and mitochondrial energy production. This assumption must be verified by animal experiment.
Type: article
URI: http://hdl.handle.net/2115/62976
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古川 貴之

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