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Hybrid origin of gynogenetic clones and the introgression of their mitochondrial genome into sexual diploids through meiotic hybridogenesis in the loach,Misgurnus anguillicuadatus

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Title: Hybrid origin of gynogenetic clones and the introgression of their mitochondrial genome into sexual diploids through meiotic hybridogenesis in the loach,Misgurnus anguillicuadatus
Authors: Yamada, Aya Browse this author
Kodo, Yukihiro Browse this author
Murakami, Masaru Browse this author
Kuroda, Masamichi Browse this author
Aoki, Takao Browse this author
Fujimoto, Takafumi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Issue Date: 14-Oct-2015
Publisher: Wiley-Blackwell
Journal Title: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume: 323
Issue: 9
Start Page: 593
End Page: 606
Publisher DOI: 10.1002/jez.1950
PMID: 26173834
Abstract: In a few Japanese populations of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae), clonal diploid lineages produce unreduced diploid eggs that normally undergo gynogenetic reproduction; however the origin of these clones remains elusive. Here, we show the presence of two diverse clades, A and B, within this loach species from sequence analyses of two nuclear genes RAG1 (recombination activating gene 1) and IRBP2 (interphotoreceptor retinoid-binding protein, 2) and then demonstrate heterozygous genotypes fixed at the two loci as the evidence of the hybrid nature of clonal lineages. All the clonal individuals were identified by clone-specific mitochondrial DNA haplotypes, microsatellite genotypes, and random amplified polymorphic DNA fingerprints; they commonly showed two alleles, one from clade A and another from clade B, whereas other wild-type diploids possessed alleles from either clade A or B. However, we also found wild-type diploids with clone-specific mitochondrial DNA and nuclear genes from clade B. One possible explanation is an introgression of a clone-specific mitochondrial genome from clonal to these wild-type loaches. These individuals likely arose by a cross between haploid sperm from bisexual B clade males and haploid eggs with clone-specific mtDNA and clade B nuclear genome, produced by meiotic hybridogenesis (elimination of unmatched A genome followed by meiosis after preferential pairing between two matched B genomes) in clone-origin triploid individual (ABB).
Rights: This is the peer reviewed version of the following article: http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291932-5231, which has been published in final form at 10.1002/jez.1950. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/63067
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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