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TARP γ-2 and γ-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area

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タイトル: TARP γ-2 and γ-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area
著者: Yamasaki, Miwako 著作を一覧する
Fukaya, Masahiro 著作を一覧する
Yamazaki, Maya 著作を一覧する
Azechi, Hirotsugu 著作を一覧する
Natsume, Rie 著作を一覧する
Abe, Manabu 著作を一覧する
Sakimura, Kenji 著作を一覧する
Watanabe, Masahiko 著作を一覧する
キーワード: AMPA receptor
hippocampus
interneuron
perforated synapse
pyramidal cell
transmembrane AMPAR regulatory proteins
発行日: 2016年 4月13日
出版者: Society for Neuroscience
誌名: Journal of neuroscience
巻: 36
号: 15
開始ページ: 4296
終了ページ: 4312
出版社 DOI: 10.1523/JNEUROSCI.4178-15.2016
抄録: The number of AMPA-type glutamate receptors (AMPARs) at synapses is the major determinant of synaptic strength and varies from synapse to synapse. To clarify the underlying molecular mechanisms, the density of AMPARs, PSD-95, and transmembrane AMPAR regulatory proteins (TARPs) were compared at Schaffer collateral/commissural (SCC) synapses in the adult mouse hippocampal CA1 by quantitative immunogold electron microscopy using serial sections. We examined four types of SCC synapses: perforated and nonperforated synapses on pyramidal cells and axodendritic synapses on parvalbumin-positive (PV synapse) and pravalbumin-negative interneurons (non-PV synapse). SCC synapses were categorized into those expressing high-density (perforated and PV synapses) or low-density (nonperforated and non-PV synapses) AMPARs. Although the density of PSD-95 labeling was fairly constant, the density and composition of TARP isoforms was highly variable depending on the synapse type. Of the three TARPs expressed in hippocampal neurons, the disparity in TARP γ-2 labeling was closely related to that of AMPAR labeling. Importantly, AMPAR density was significantly reduced at perforated and PV synapses in TARP γ-2-knock-out (KO) mice, resulting in a virtual loss of AMPAR disparity among SCC synapses. In comparison, TARP γ-8 was the only TARP expressed at nonperforated synapses, where AMPAR labeling further decreased to a background level in TARP γ-8-KO mice. These results show that synaptic inclusion of TARP γ-2 potently increases AMPAR expression and transforms low-density synapses into high-density ones, whereas TARP γ-8 is essential for low-density or basal expression of AMPARs at nonperforated synapses. Therefore, these TARPs are critically involved in AMPAR density control at SCC synapses.
資料タイプ: article
URI: http://hdl.handle.net/2115/63068
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山崎 美和子

 

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