|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell |
| Authors: | Oura, Makoto Browse this author | | Yamamoto, Johtaro Browse this author | | Ishikawa, Hideto Browse this author | | Mikuni, Shintaro Browse this author | | Fukushima, Ryousuke Browse this author | | Kinjo, Masataka Browse this author |
| Issue Date: | 5-Aug-2016 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 6 |
| Start Page: | 31091 |
| Publisher DOI: | 10.1038/srep31091 |
| Abstract: | Rotational diffusion measurement is predicted as an important method in cell biology because the rotational properties directly reflect molecular interactions and environment in the cell. To prove this concept, polarization-dependent fluorescence correlation spectroscopy (pol-FCS) measurements of purified fluorescent proteins were conducted in viscous solution. With the comparison between the translational and rotational diffusion coefficients obtained from pol-FCS measurements, the hydrodynamic radius of an enhanced green fluorescent protein (EGFP) was estimated as a control measurement. The orientation of oligomer EGFP in living cells was also estimated by pol-FCS and compared with Monte Carlo simulations. The results of this pol-FCS experiment indicate that this method allows an estimation of the molecular orientation using the characteristics of rotational diffusion. Further, it can be applied to analyze the degree of molecular orientation and multimerization or detection of tiny aggregation of aggregate-prone proteins. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63083 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 金城 政孝
|
