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Structure-property relationships of photoresponsive inhibitors of the kinesin motor
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| Title: | Structure-property relationships of photoresponsive inhibitors of the kinesin motor |
| Authors: | Amrutha, Ammathnadu S. Browse this author | | Kumar, K. R. Sunil Browse this author | | Matsuo, Kazuya Browse this author | | Tamaoki, Nobuyuki Browse this author →KAKEN DB |
| Issue Date: | 14-Aug-2016 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Organic & Biomolecular Chemistry |
| Volume: | 14 |
| Issue: | 30 |
| Start Page: | 7202 |
| End Page: | 7210 |
| Publisher DOI: | 10.1039/c6ob00951d |
| PMID: | 27270305 |
| Abstract: | Recently we demonstrated the photoregulation of the activity of kinesin-1 using an azobenzene-tethered peptide (azo-peptide: Azo-Ile-Pro-Lys-Ala-Ile-Gln-Ala-Ser-His-Gly-Arg-OH). To understand the mechanism behind this photoswitchable inhibition, here we studied the structure-property relationships of a range of azo-peptides through systematic variations in the structures of the peptide and azobenzene units. The vital peptide sequence for kinesin inhibition-mediated through electrostatic, hydrophobic and C-H center dot center dot center dot pi interactions-was the same as that for the self-inhibition of kinesin. We also identified substituents on the azobenzene capable of enhancing the photoswitchability of inhibition. As a result, we developed a new inhibitor featuring a relatively short peptide unit (-Arg-Ile-Pro-Lys-Ala-Ile-Arg-OH) and an azobenzene unit bearing a para-OMe group. In the trans form of its azobenzene unit, this finely tuned inhibitor stopped the kinesin-driven gliding motility of microtubules completely at a relatively low concentration, yet allowed gliding motility with a relatively high velocity in the cis form obtained after UV irradiation. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63139 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 玉置 信之
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