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Swim bladder collagen forms hydrogel with macroscopic superstructure by diffusion induced fast gelation

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この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/63152

タイトル: Swim bladder collagen forms hydrogel with macroscopic superstructure by diffusion induced fast gelation
著者: Mredha, Md. Tariful Islam 著作を一覧する
Zhang, Xi 著作を一覧する
Nonoyama, Takayuki 著作を一覧する
Nakajima, Tasuku 著作を一覧する
Kurokawa, Takayuki 著作を一覧する
Takagid, Yasuaki 著作を一覧する
Gong, Jian Ping 著作を一覧する
発行日: 2015年10月21日
出版者: Royal Society of Chemistry
誌名: Journal of materials chemistry b
巻: 3
号: 39
開始ページ: 7658
終了ページ: 7666
出版社 DOI: 10.1039/c5tb00877h
抄録: Marine collagen has been attracting attention as a medical material in recent times due to the low risk of pathogen infection compared to animal collagen. Type I collagen extracted from the swim bladder of Bester sturgeon fish has excellent characteristics such as high denaturation temperature, high solubility, low viscosity and an extremely fast rate to form large bundle of fibers under certain conditions. These specific characteristics of swim bladder collagen (SBC) permit us to create stable, disk shaped hydrogels with concentric orientation of collagen fibers by the controlled diffusion of neutral buffer through collagen solution at room temperature. However, traditionally used animal collagens, e.g. calf skin collagen (CSC) and porcine skin collagen (PSC), could not form any stable and oriented structure by this method. The mechanism of the superstructure formation of SBC by a diffusion induced gelation process has been explored. The fast fibrillogenesis rate of SBC causes a quick squeezing out of the solvent from the gel phase to the sol phase during gelation, which builds an internal stress at the gel-sol interface. The tensile stress induces the collagen molecules of the gel phase to align along the gel-sol interface direction to give this concentric ring-shaped orientation pattern. On the other hand, the slow fibrillogenesis rate of animal collagens due to the high viscosity of the solution does not favor the ordered structure formation. The denaturation temperature of SBC increases significantly from 31 degrees C to 43 degrees C after gelation, whereas that of CSC and PSC were found to increase a little. Rheology experiment shows that the SBC gel has storage modulus larger than 15 kPa. The SBC hydrogels with thermal and mechanical stability have potential as bio-materials for tissue engineering applications.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/63152
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 龔 剣萍 (Gong Jian Ping)

 

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