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In Vivo Mouse Intervertebral Disc Degeneration Model Based on a New Histological Classification

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Title: In Vivo Mouse Intervertebral Disc Degeneration Model Based on a New Histological Classification
Authors: Ohnishi, Takashi Browse this author
Sudo, Hideki Browse this author →KAKEN DB
Iwasaki, Koji Browse this author
Tsujimoto, Takeru Browse this author
Ito, Yoichi M. Browse this author →KAKEN DB
Iwasaki, Norimasa Browse this author →KAKEN DB
Issue Date: 2-Aug-2016
Publisher: The Public Library of Science
Journal Title: PLoS ONE
Volume: 11
Issue: 8
Start Page: e0160486
Publisher DOI: 10.1371/journal.pone.0160486
Abstract: Although human intervertebral disc degeneration can lead to several spinal diseases, its pathogenesis remains unclear. This study aimed to create a new histological classification applicable to an in vivo mouse intervertebral disc degeneration model induced by needle puncture. One hundred six mice were operated and the L4/5 intervertebral disc was punctured with a 35- or 33-gauge needle. Micro-computed tomography scanning was performed, and the punctured region was confirmed. Evaluation was performed by using magnetic resonance imaging and histology by employing our classification scoring system. Our histological classification scores correlated well with the findings of magnetic resonance imaging and could detect degenerative progression, irrespective of the punctured region. However, the magnetic resonance imaging analysis revealed that there was no significant degenerative intervertebral disc change between the ventrally punctured and non-punctured control groups. To induce significant degeneration in the lumbar intervertebral discs, the central or dorsal region should be punctured instead of the ventral region.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 須藤 英毅

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