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GluD2 Endows Parallel Fiber-Purkinje Cell Synapses with a High Regenerative Capacity

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Title: GluD2 Endows Parallel Fiber-Purkinje Cell Synapses with a High Regenerative Capacity
Authors: Ichikawa, Ryoichi Browse this author →KAKEN DB
Sakimura, Kenji Browse this author →KAKEN DB
Watanabe, Masahiko Browse this author →KAKEN DB
Keywords: cerebellum
climbing fiber
parallel fiber
Purkinje cell
synapse regeneration
Issue Date: 27-Apr-2016
Publisher: Society for Neuroscience
Journal Title: Journal of neuroscience
Volume: 36
Issue: 17
Start Page: 4846
End Page: 4858
Publisher DOI: 10.1523/JNEUROSCI.0161-16.2016
PMID: 27122040
Abstract: Although injured axons usually do not regenerate in the adult CNS, parallel fibers (PFs) regenerate synaptic connections onto cerebellar Purkinje cells (PCs). In this study, we investigated the role of GluD2 in this regenerative process after PF transection using GluD2-knock-out (KO) mice. All dendritic spines on distal dendrites were innervated by PFs in sham-operated wild-type controls, whereas one-third were devoid of innervation in GluD2-KO mice. In both genotypes, a steep drop in the number of PF synapses occurred with a reciprocal surge in the number of free spines on postlesion day 1, when the PF territory aberrantly expanded toward the proximal dendrites. In wild-type mice, the territory and number of PF synapses were nearly fully restored to normal on postlesion day 7, although PF density remained low. Moreover, presynaptic and postsynaptic elements were markedly enlarged, and the PF terminal-to-PC spine contact ratio increased from 1:1 to 1:2 at most synapses. On postlesion day 30, the size and contact ratio of PF synapses returned to sham-operated control values and PF density recovered through the sprouting and elongation of PF collaterals. However, GluD2-KO mice showed neither a hypertrophic response nor territorial restoration 7 d postlesion, nor the recovery of PF axons or synapses on postlesion day 30. This suggests that PF wiring regenerates initially by inducing hypertrophic responses in surviving synaptic elements (hypertrophic phase), followed by collateral formation by PF axons and retraction of PF synapses (remodeling phase). Without GluD2, no transition to these regenerative phases occurs.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉 雅彦

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