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Localization of Minodronate in Mouse Femora Through Isotope Microscopy

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タイトル: Localization of Minodronate in Mouse Femora Through Isotope Microscopy
その他のタイトル: Localization of minodronate in bone
著者: Hongo, Hiromi 著作を一覧する
Sasaki, Muneteru 著作を一覧する
Kobayashi, Sachio 著作を一覧する
Hasegawa, Tomoka 著作を一覧する
Yamamoto, Tomomaya 著作を一覧する
Tsuboi, Kanako 著作を一覧する
Tsuchiya, Erika 著作を一覧する
Nagai, Tomoya 著作を一覧する
Khadiza, Naznin 著作を一覧する
Abe, Miki 著作を一覧する
Kudo, Ai 著作を一覧する
Oda, Kimimitsu 著作を一覧する
Henrique Luiz de Freitas, Paulo 著作を一覧する
Li, Minqi 著作を一覧する
Yurimoto, Hisayoshi 著作を一覧する
Amizuka, Norio 著作を一覧する
キーワード: minodronate
osteoclast
osteoblast
isotope microscopy
bisphosphonate
発行日: 2016年10月
出版者: SAGE Publications
誌名: Journal of Histochemistry & Cytochemistry
巻: 64
号: 10
開始ページ: 601
終了ページ: 622
出版社 DOI: 10.1369/0022155416665577
抄録: Minodronate is highlighted for its marked and sustained effects on osteoporotic bones. To determine the duration of minodronate’s effects, we have assessed the localization of the drug in mouse bones through isotope microscopy, after labeling it with a stable nitrogen isotope (15N-minodronate). In addition, minodronate-treated bones were assessed by histochemistry and TEM. Eight-weeks-old male ICR mice received 15N-minodronate (1mg/kg) intravenously and were sacrificed after three hours, 24 hrs, one week and one month. Isotope microscopy showed that 15N-minodronate was present mainly beneath osteoblasts rather than nearby osteoclasts. At 3 hrs after minodronate administration, histochemistry and TEM showed osteoclasts with well-developed ruffled borders. However, osteoclasts were roughly attached to the bone surfaces and did not feature ruffled borders at 24 hrs after minodronate administration. The numbers of TRAP-positive osteoclasts and ALP-reactive osteoblastic area were not reduced suddenly, and apoptotic osteoclasts appeared in 1 week and 1 month after the injections. Von Kossa staining demonstrated that osteoclasts treated with minodronate did not incorporate mineralized bone matrix. Taken together, minodronate accumulates in bone underneath osteoblasts rather than under bone-resorbing osteoclasts; therefore, it is likely that the minodronate-coated bone matrix is resistant to osteoclastic resorption, which results in a long-lasting and bone-preserving effect.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/63234
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 網塚 憲生

 

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