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Sulfurifustis variabilis gen. nov., sp nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov and Acidiferrobacterales ord. nov.
Title: | Sulfurifustis variabilis gen. nov., sp nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov and Acidiferrobacterales ord. nov. |
Authors: | Kojima, Hisaya Browse this author | Shinohara, Arisa Browse this author | Fukui, Manabu Browse this author |
Issue Date: | 30-Oct-2015 |
Publisher: | Society for General Microbiology |
Journal Title: | International journal of systematic and evolutionary microbiology |
Volume: | 65 |
Start Page: | 3709 |
End Page: | 3713 |
Publisher DOI: | 10.1099/ijsem.0.000479 |
PMID: | 26220671 |
Abstract: | A novel autotrophic bacterium, strain skN76(T), was isolated from sediment of a lake in Japan. As sole electron donor to support chemolithoautotrophic growth, the strain oxidized thiosulfate, tetrathionate and elemental sulfur. For growth, the optimum temperature was 42-45 degrees C and the optimum pH was 6.8-8.2. The cells were Gram-stain-negative, catalase-positive and oxidase-positive. The strain exhibited changes in morphology depending on growth temperature. Cells grown at the optimum temperature were rod-shaped (0.9-3.0 mu m long and 0.3-0.5 mu m wide), whereas a filamentous form was observed when the strain was cultured at the lowest permissive growth temperatures. The G+C content of genomic DNA was 69 mol%. The major components in the fatty acid profile were C-16 : 0, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and summed feature 9 (iso-C-17 : 1 omega 9c and/or 10-methyl C-16 : 0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest cultivated relative of strain skN76(T) was Acidiferrobacter thiooxydans m-1(T), with sequence similarity of 93 %. On the basis of its phylogenetic and phenotypic properties, strain skN76(T) (=DSM 100313(T)= NBRC 110942(T)) is proposed as the type strain of a novel species of a novel genus, Sulfurifustis variabilis gen. nov., sp. nov. Novel taxa, Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov., are also proposed to accommodate the genera Acidiferrobacter and Sulfurifustis gen. nov. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/63357 |
Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小島 久弥
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