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Sulfurifustis variabilis gen. nov., sp nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov and Acidiferrobacterales ord. nov.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/63357

Title: Sulfurifustis variabilis gen. nov., sp nov., a sulfur oxidizer isolated from a lake, and proposal of Acidiferrobacteraceae fam. nov and Acidiferrobacterales ord. nov.
Authors: Kojima, Hisaya Browse this author
Shinohara, Arisa Browse this author
Fukui, Manabu Browse this author
Issue Date: 30-Oct-2015
Publisher: Society for General Microbiology
Journal Title: International journal of systematic and evolutionary microbiology
Volume: 65
Start Page: 3709
End Page: 3713
Publisher DOI: 10.1099/ijsem.0.000479
PMID: 26220671
Abstract: A novel autotrophic bacterium, strain skN76(T), was isolated from sediment of a lake in Japan. As sole electron donor to support chemolithoautotrophic growth, the strain oxidized thiosulfate, tetrathionate and elemental sulfur. For growth, the optimum temperature was 42-45 degrees C and the optimum pH was 6.8-8.2. The cells were Gram-stain-negative, catalase-positive and oxidase-positive. The strain exhibited changes in morphology depending on growth temperature. Cells grown at the optimum temperature were rod-shaped (0.9-3.0 mu m long and 0.3-0.5 mu m wide), whereas a filamentous form was observed when the strain was cultured at the lowest permissive growth temperatures. The G+C content of genomic DNA was 69 mol%. The major components in the fatty acid profile were C-16 : 0, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and summed feature 9 (iso-C-17 : 1 omega 9c and/or 10-methyl C-16 : 0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest cultivated relative of strain skN76(T) was Acidiferrobacter thiooxydans m-1(T), with sequence similarity of 93 %. On the basis of its phylogenetic and phenotypic properties, strain skN76(T) (=DSM 100313(T)= NBRC 110942(T)) is proposed as the type strain of a novel species of a novel genus, Sulfurifustis variabilis gen. nov., sp. nov. Novel taxa, Acidiferrobacteraceae fam. nov. and Acidiferrobacterales ord. nov., are also proposed to accommodate the genera Acidiferrobacter and Sulfurifustis gen. nov.
Type: article (author version)
URI: http://hdl.handle.net/2115/63357
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小島 久弥

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