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Hokkaido University Collection of Scholarly and Academic Papers >
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Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus
| Title: | Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus |
| Authors: | Yoshikawa, Tomoko Browse this author →KAKEN DB | | Nakajima, Yoshihiro Browse this author →KAKEN DB | | Yamada, Yoshiko Browse this author →KAKEN DB | | Enoki, Ryosuke Browse this author →KAKEN DB | | Watanabe, Kazuto Browse this author →KAKEN DB | | Yamazaki, Maya Browse this author →KAKEN DB | | Sakimura, Kenji Browse this author →KAKEN DB | | Honma, Sato Browse this author →KAKEN DB | | Honma, Ken-ichi Browse this author →KAKEN DB |
| Keywords: | arginine vasopressin-Emerald-luciferase | | bioluminescence | | circadian rhythm | | mouse | | suprachiasmatic nucleus | | AVP-ELuc | | SCN |
| Issue Date: | Nov-2015 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | European journal of neuroscience |
| Volume: | 42 |
| Issue: | 9 |
| Start Page: | 2678 |
| End Page: | 2689 |
| Publisher DOI: | 10.1111/ejn.13061 |
| PMID: | 26342201 |
| Abstract: | Arginine vasopressin (AVP), a major neuropeptide in the suprachiasmatic nucleus (SCN), is postulated to mediate the output of the circadian oscillation. Mice carrying a reporter gene of AVP transcription (AVP(ELuc)) were produced by knocking-in a cDNA of Emerald-luciferase (ELuc) in the translational initiation site. Homozygous mice did not survive beyond postnatal day 7. Using the heterozygous (AVP(ELuc/+)) mice, a bioluminescence reporter system was developed that enabled to monitor AVP transcription through AVP-ELuc measurement in real time for more than 10 cycles in the cultured brain slice. AVP(ELuc/+) mice showed circadian behaviour rhythms and light responsiveness indistinguishable from those of the wild-type. Robust circadian rhythms in AVP-ELuc were detected in the cultured SCN slice at a single cell as well as tissue levels. The circadian rhythm of the whole SCN slice was stable, with the peak at the mid-light phase of a light-dark cycle, while that of a single cell was more variable. By comparison, rhythmicity in the paraventricular nucleus and supraoptic nucleus in the hypothalamus was unstable and damped rapidly. Spatiotemporal profiles of AVP expression at the pixel level revealed significant circadian rhythms in the entire area of AVP-positive cells in the SCN, and at least two clusters that showed different circadian oscillations. Contour analysis of bioluminescence intensity in a cell-like region demonstrated the radiation area was almost identical to the cell size. This newly developed reporter system for AVP gene expression is a useful tool for the study of circadian rhythms. |
| Rights: | This is the peer reviewed version of the following article: Yoshikawa, T., Nakajima, Y., Yamada, Y., Enoki, R., Watanabe, K., Yamazaki, M., Sakimura, K., Honma, S., Honma, K.-i. (2015), Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus. European Journal of Neuroscience, 42: 2678–2689., which has been published in final form at http://dx.doi.org/10.1111/ejn.13061. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/63362 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉川 朋子
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