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Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus

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Title: Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus
Authors: Yoshikawa, Tomoko Browse this author →KAKEN DB
Nakajima, Yoshihiro Browse this author →KAKEN DB
Yamada, Yoshiko Browse this author →KAKEN DB
Enoki, Ryosuke Browse this author →KAKEN DB
Watanabe, Kazuto Browse this author →KAKEN DB
Yamazaki, Maya Browse this author →KAKEN DB
Sakimura, Kenji Browse this author →KAKEN DB
Honma, Sato Browse this author →KAKEN DB
Honma, Ken-ichi Browse this author →KAKEN DB
Keywords: arginine vasopressin-Emerald-luciferase
circadian rhythm
suprachiasmatic nucleus
Issue Date: Nov-2015
Publisher: Wiley-Blackwell
Journal Title: European journal of neuroscience
Volume: 42
Issue: 9
Start Page: 2678
End Page: 2689
Publisher DOI: 10.1111/ejn.13061
PMID: 26342201
Abstract: Arginine vasopressin (AVP), a major neuropeptide in the suprachiasmatic nucleus (SCN), is postulated to mediate the output of the circadian oscillation. Mice carrying a reporter gene of AVP transcription (AVP(ELuc)) were produced by knocking-in a cDNA of Emerald-luciferase (ELuc) in the translational initiation site. Homozygous mice did not survive beyond postnatal day 7. Using the heterozygous (AVP(ELuc/+)) mice, a bioluminescence reporter system was developed that enabled to monitor AVP transcription through AVP-ELuc measurement in real time for more than 10 cycles in the cultured brain slice. AVP(ELuc/+) mice showed circadian behaviour rhythms and light responsiveness indistinguishable from those of the wild-type. Robust circadian rhythms in AVP-ELuc were detected in the cultured SCN slice at a single cell as well as tissue levels. The circadian rhythm of the whole SCN slice was stable, with the peak at the mid-light phase of a light-dark cycle, while that of a single cell was more variable. By comparison, rhythmicity in the paraventricular nucleus and supraoptic nucleus in the hypothalamus was unstable and damped rapidly. Spatiotemporal profiles of AVP expression at the pixel level revealed significant circadian rhythms in the entire area of AVP-positive cells in the SCN, and at least two clusters that showed different circadian oscillations. Contour analysis of bioluminescence intensity in a cell-like region demonstrated the radiation area was almost identical to the cell size. This newly developed reporter system for AVP gene expression is a useful tool for the study of circadian rhythms.
Rights: This is the peer reviewed version of the following article: Yoshikawa, T., Nakajima, Y., Yamada, Y., Enoki, R., Watanabe, K., Yamazaki, M., Sakimura, K., Honma, S., Honma, K.-i. (2015), Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus. European Journal of Neuroscience, 42: 2678–2689., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉川 朋子

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