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Effects of human amnion-derived mesenchymal stromal cell transplantation in rats with radiation proctitis

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タイトル: Effects of human amnion-derived mesenchymal stromal cell transplantation in rats with radiation proctitis
著者: Ono, Masayoshi 著作を一覧する
Ohnishi, Shunsuke 著作を一覧する
Honda, Minori 著作を一覧する
Ishikawa, Marin 著作を一覧する
Hosono, Hidetaka 著作を一覧する
Onishi, Reizo 著作を一覧する
Nakagawa, Koji 著作を一覧する
Takeda, Hiroshi 著作を一覧する
Sakamoto, Naoya 著作を一覧する
キーワード: amnion
cell injury
mesenchymal stromal cells
radiation proctitis
発行日: 2015年11月
出版者: Elsevier
誌名: Cytotherapy
巻: 17
号: 11
開始ページ: 1545
終了ページ: 1559
出版社 DOI: 10.1016/j.jcyt.2015.07.003
抄録: Background aims. Mesenchymal stromal cells (MSCs) have been reported to be a promising cell source in cell therapy, and large amounts of MSCs can easily be isolated from human amnion. Therapeutic irradiation for intra-pelvic cancer often causes radiation proctitis; however, there is currently no effective treatment. We therefore investigated the effect of transplantation of human amnion derived MSCs (AMSCs) in rats with radiation proctitis. Methods. Amnion was obtained at cesarean delivery, and AMSCs were isolated and expanded. Sprague-Dawley rats were gamma-irradiated (5 Gy/d) at the rectum for 5 days. On day 5, AMSCs (1 x 10(6) cells) were intravenously transplanted. Rats were killed on day 8. Histological analyses were performed, and messenger RNA expression of inflammatory mediators was measured. In vitro, after gamma-irradiation of rat intestinal epithelial cells (IEC-6), the cells were cultured with AMSC-conditioned medium (CM). The effect of AMSC-CM was evaluated by measurement of caspase-3/7 activity, p53 transcription activity and quantitative reverse-transcription polymerase chain reaction for p53-target genes. Results. Histological examination demonstrated that epithelial injury and infiltration of inflammatory cells in the rectum were significantly suppressed by transplantation of AMSCs. In vitro, the cell injury in IEC-6 cells induced by gamma-irradiation was inhibited by AMSC-CM, which also inhibited the upregulation of p53 transcription activity, caspase-3/7 activity and p21 expression. Conclusions. Transplantation of AMSCs improved radiation proctitis, possibly through inhibition of cell injury and inflammatory reactions. AMSC transplantation should be considered as a new treatment for radiation proctitis.
Rights: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/63375
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大西 俊介

 

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