HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >

A small scale study on the effects of oral administration of the β-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves

Creative Commons License

Files in This Item:
art_10.1186_1756-0500-5-189.pdf1.06 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/63406

Title: A small scale study on the effects of oral administration of the β-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves
Authors: Uchiyama, Hirofumi Browse this author
Iwai, Atsushi Browse this author
Asada, Yukoh Browse this author
Muramatsu, Daisuke Browse this author
Aoki, Shiho Browse this author
Kawata, Koji Browse this author
Kusano, Kisato Browse this author
Nagashima, Koji Browse this author
Yasokawa, Daisuke Browse this author
Okabe, Mitsuyasu Browse this author
Miyazaki, Tadaaki Browse this author →KAKEN DB
Issue Date: 25-Apr-2012
Publisher: BioMed Central
Journal Title: BMC Research Notes
Volume: 5
Issue: 1
Start Page: 189
Publisher DOI: 10.1186/1756-0500-5-189
Abstract: Background: The β–(1→3),(1→6)-D-glucan extracellularly produced by Aureobasidium pullulans exhibits immunomodulatory activity, and is used for health supplements. To examine the effects of oral administration of the β–(1→3),(1→6)-D-glucan to domestic animals, a small scale study was conducted using Holstein cows and newborn Japanese Black calves. Findings: Holstein cows of which somatic cell count was less than 3 x 105/ml were orally administered with or without the β-(1→3),(1→6)-D-glucan-enriched A. pullulans cultured fluid (AP-CF) for 3 months, and the properties of milk and serum cytokine expression were monitored. Somatic cell counts were not significantly changed by oral administration of AP-CF, whereas the concentration of solid non fat in the milk tended to increase in the AP-CF administered cows. The results of cytokine expression analysis in the serum using ELISA indicate that the expressions of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in all cows which were orally administered with AP-CF became slightly lower than that of control cows after the two-month treatment. On the other hand, IL-8 expression tended to indicate a moderately higher level in all treated cows after the three-month administration of AP-CF in comparison with that of the control cows. Peripartum Japanese Black beef cows and their newborn calves were orally administered with AP-CF, and bacterial flora in the intestines of the calves were analyzed by T-RFLP (terminal restriction fragment length polymorphism). The results suggest that bacterial flora are tendentiously changed by oral administration of AP-CF. Conclusions: Our data indicated the possibility that oral administration of the β–(1→3),(1→6)-D- glucan produced by A. pullulans affects cytokine expressions in the serum of Holstein cows, and influences bacterial flora in the intestines of Japanese Black calves. The findings may be helpful for further study on the efficacies of oral administration of β-(1→3),(1→6)-D-glucans on domestic animals.
Rights: http://creativecommons.org/licenses/by/2.0
Type: article
URI: http://hdl.handle.net/2115/63406
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岩井 淳

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University