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Stimulation of Macrophages with the β-Glucan Produced by Aureobasidium pullulans Promotes the Secretion of Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)

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タイトル: Stimulation of Macrophages with the β-Glucan Produced by Aureobasidium pullulans Promotes the Secretion of Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)
著者: Kawata, Koji 著作を一覧する
Iwai, Atsushi 著作を一覧する
Muramatsu, Daisuke 著作を一覧する
Aoki, Shiho 著作を一覧する
Uchiyama, Hirofumi 著作を一覧する
Okabe, Mitsuyasu 著作を一覧する
Hayakawa, Sumio 著作を一覧する
Takaoka, Akinori 著作を一覧する
Miyazaki, Tadaaki 著作を一覧する
発行日: 2015年 4月13日
出版者: The Public Library of Science (PLoS)
誌名: PLOS ONE
巻: 10
号: 4
開始ページ: e0124809
出版社 DOI: 10.1371/journal.pone.0124809
抄録: A β-glucan produced by Aureobasidium pullulans (AP-PG) is consisting of a β-(1,3)-linked main chain with β-(1,6)-linked glucose side residues. Various β-glucans consisting of β-(1,3)-linked main chain including AP-PG are believed to exhibit anti-tumor activities, and actually, anti-tumor activities of AP-PG in mice have been demonstrated. In this study, we demonstrate that stimulation with AP-PG induces TRAIL expression in mouse and human macrophage-like cell lines. TRAIL is known to be a cytokine which specifically induces apoptosis in transformed cells, but not in untransformed cells. The expression of TRAIL mRNA after stimulation with AP-PG was increased in RAW264.7 cells, Mono Mac 6 cells, and macrophage-differentiated THP-1 cells. The mRNA expression of TNF-α and FasL is only weakly increased after stimulation with AP-PG. The induction activity of TRAIL by curdlan, a bacterial β-glucan, was very similar to that by AP-PG in RAW264.7 cells, but weaker in macrophage-differentiated THP-1 cells. Activation of caspases was found in HeLa cells after treatment with the supernatant of cultured medium from AP-PG-stimulated Mono Mac 6 cells, and was inhibited by the anti-TRAIL neutralizing antibody. These findings suggest that the stimulation with AP-PG effectively induces TRAIL in macrophages, and that it may be related to apoptosis induction of tumor cells.
資料タイプ: article
URI: http://hdl.handle.net/2115/63433
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 岩井 淳

 

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