| Title: | The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice |
| Authors: | Furihata, Takaaki Browse this author →KAKEN DB |
| Kinugawa, Shintaro Browse this author →KAKEN DB |
| Takada, Shingo Browse this author →KAKEN DB |
| Fukushima, Arata Browse this author →KAKEN DB |
| Takahashi, Masashige Browse this author |
| Homma, Tsuneaki Browse this author |
| Masaki, Yoshihiro Browse this author |
| Tsuda, Masaya Browse this author |
| Matsumoto, Junichi Browse this author |
| Mizushima, Wataru Browse this author |
| Matsushima, Shouji Browse this author |
| Yokota, Takashi Browse this author →KAKEN DB |
| Tsutsui, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | Methodology |
| Transverse aortic constriction |
| Cardiac hypertrophy |
| Cardiac failure |
| Mice |
| Issue Date: | Jun-2016 |
| Publisher: | Elsevier |
| Journal Title: | IJC Heart & Vasculature |
| Volume: | 11 |
| Start Page: | 24 |
| End Page: | 28 |
| Publisher DOI: | 10.1016/j.ijcha.2016.03.007 |
| Abstract: | Background: Transverse aortic constriction (TAC) operation is used as an experimental model of left ventricular (LV) hypertrophy and LV failure in mice. The severity of LV remodeling or failure may depend on the degree of TAC, but is variable among operated animals. Therefore,we tried to identify the optimal diameter of TAC to create this model with ease and high reproducibility. Methods and results: To produce TAC in C57BL/6J mice (7-9 weeks, body weight 19-26 g, n = 109), a 7-0 nylon suture ligature was tightly tied around the transverse aorta against needles with 3 different diameters (mm); 0.40, 0.385 and 0.375. LVwall thickness, end-diastolic dimension, fractional shorteningweremeasured by echocardiography. At 4 weeks after TAC, no mouse with the 0.400 mm gauge progressed in LV failure. The 0.385 mm pin gauge mouse kept a more survival rate compared with the 0.375 mm (59% vs 48%), representing same efficient in LV failure.With the 0.385mmpin gauge, hearts of mice remained LV hypertrophy at 1 week after TAC, followed by LV failure at 4 weeks. Conclusion: TACwith the diameter of 0.385mmcan effectively induce the transition from LV hypertrophy to failure in mice with relatively preserved survival. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63446 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
