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The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice

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Title: The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice
Authors: Furihata, Takaaki Browse this author →KAKEN DB
Kinugawa, Shintaro Browse this author →KAKEN DB
Takada, Shingo Browse this author →KAKEN DB
Fukushima, Arata Browse this author →KAKEN DB
Takahashi, Masashige Browse this author
Homma, Tsuneaki Browse this author
Masaki, Yoshihiro Browse this author
Tsuda, Masaya Browse this author
Matsumoto, Junichi Browse this author
Mizushima, Wataru Browse this author
Matsushima, Shouji Browse this author
Yokota, Takashi Browse this author →KAKEN DB
Tsutsui, Hiroyuki Browse this author →KAKEN DB
Keywords: Methodology
Transverse aortic constriction
Cardiac hypertrophy
Cardiac failure
Issue Date: Jun-2016
Publisher: Elsevier
Journal Title: IJC Heart & Vasculature
Volume: 11
Start Page: 24
End Page: 28
Publisher DOI: 10.1016/j.ijcha.2016.03.007
Abstract: Background: Transverse aortic constriction (TAC) operation is used as an experimental model of left ventricular (LV) hypertrophy and LV failure in mice. The severity of LV remodeling or failure may depend on the degree of TAC, but is variable among operated animals. Therefore,we tried to identify the optimal diameter of TAC to create this model with ease and high reproducibility. Methods and results: To produce TAC in C57BL/6J mice (7-9 weeks, body weight 19-26 g, n = 109), a 7-0 nylon suture ligature was tightly tied around the transverse aorta against needles with 3 different diameters (mm); 0.40, 0.385 and 0.375. LVwall thickness, end-diastolic dimension, fractional shorteningweremeasured by echocardiography. At 4 weeks after TAC, no mouse with the 0.400 mm gauge progressed in LV failure. The 0.385 mm pin gauge mouse kept a more survival rate compared with the 0.375 mm (59% vs 48%), representing same efficient in LV failure.With the 0.385mmpin gauge, hearts of mice remained LV hypertrophy at 1 week after TAC, followed by LV failure at 4 weeks. Conclusion: TACwith the diameter of 0.385mmcan effectively induce the transition from LV hypertrophy to failure in mice with relatively preserved survival.
Type: article
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 降旗 高明

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