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Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation
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Title: | Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation |
Authors: | Kimura, Shunsuke Browse this author →KAKEN DB | Yamashita, Masami Browse this author | Yamakami-Kimura, Megumi Browse this author | Sato, Yusuke Browse this author | Yamagata, Atsushi Browse this author →KAKEN DB | Kobashigawa, Yoshihiro Browse this author | Inagaki, Fuyuhiko Browse this author →KAKEN DB | Amada, Takako Browse this author | Hase, Koji Browse this author →KAKEN DB | Iwanaga, Toshihiko Browse this author →KAKEN DB | Ohno, Hiroshi Browse this author →KAKEN DB | Fukai, Shuya Browse this author →KAKEN DB |
Issue Date: | 15-Sep-2016 |
Publisher: | Nature Publishing Group |
Journal Title: | Scientific reports |
Volume: | 6 |
Start Page: | 33548 |
Publisher DOI: | 10.1038/srep33548 |
Abstract: | The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/63450 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 俊介
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