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Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation

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Title: Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation
Authors: Kimura, Shunsuke Browse this author →KAKEN DB
Yamashita, Masami Browse this author
Yamakami-Kimura, Megumi Browse this author
Sato, Yusuke Browse this author
Yamagata, Atsushi Browse this author →KAKEN DB
Kobashigawa, Yoshihiro Browse this author
Inagaki, Fuyuhiko Browse this author →KAKEN DB
Amada, Takako Browse this author
Hase, Koji Browse this author →KAKEN DB
Iwanaga, Toshihiko Browse this author →KAKEN DB
Ohno, Hiroshi Browse this author →KAKEN DB
Fukai, Shuya Browse this author →KAKEN DB
Issue Date: 15-Sep-2016
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 6
Start Page: 33548
Publisher DOI: 10.1038/srep33548
Abstract: The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 俊介

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