HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Files in This Item:
Published ijmm_36_2_369_PDF.pdf1.7 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/63492

Title: Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death
Authors: Choi, Youn Browse this author
Kim, Eun-Young Browse this author
Mikami, Koji Browse this author →KAKEN DB
Nam, Taek Browse this author
Keywords: Pyropia yezoensis
recombinant protein
chemoprotective effect
acetaminophen-induced cell death
Issue Date: Aug-2015
Publisher: Spandidos Publications
Journal Title: International Journal of Molecular Medicine
Volume: 36
Issue: 2
Start Page: 369
End Page: 376
Publisher DOI: 10.3892/ijmm.2015.2253
Abstract: In the present study, the chemoprotective effects of recombinant Pyropia yezoensis (P. yezoensis) protein 1 (PYP1) were examined in acetaminophen (APAP)-treated Chang liver cells. The analysis of P. yezoensis revealed the presence of both mature and immature variants of PYP1. PYP1s, designated as PYP1 (15 kDa), PYP1-AC (12 kDa) and PYP1-B (5 kDa), were successfully expressed in Escherichia coli, and their chemoprotective effects were then examined. In addition, a peptide of 11 residues (ALEGGKSSGGG), which is a common sequence at the N-terminus all of the PYP1s, was synthesized and examined. The effects of treatment with PYP1s and the synthetic peptide (SP) on cell proliferation were determined by MTS assay. Our results clearly demonstrated that treatment with all the PYP1s and SP significantly promoted the proliferation of Chang liver cells, protecting them against APAP. Thus, we concluded that recombinant PYP1s exert protective effects against injury to Chang liver cells.
Relation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501640/
Type: article
URI: http://hdl.handle.net/2115/63492
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University