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Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.)

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Title: Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.)
Authors: Kagami, Hiroyo Browse this author
Taguchi, Kazunori Browse this author
Arakawa, Takumi Browse this author
Kuroda, Yosuke Browse this author
Tamagake, Hideto Browse this author
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: Dedifferentiation
DNA marker
F1 hybrid
in vitro culture
Somatic embryo
Issue Date: 15-Nov-2016
Publisher: BioMed Central
Journal Title: Hereditas
Volume: 153
Publisher DOI: 10.1186/s41065-016-0015-z
Abstract: Background: Obtaining dedifferentiated cells (callus) that can regenerate into whole plants is not always feasible for many plant species. Sugar beet is known to be recalcitrant for dedifferentiation and plant regeneration. These difficulties were major obstacles for obtaining transgenic sugar beets through an Agrobacterium-mediated transformation procedure. The sugar beet line ‘NK-219mm-O’ is an exceptional line that forms callus efficiently and is easy to regenerate, but the inheritance of these characters was unknown. Another concern was whether these characters could coexist with an annual habitat that makes it possible to breed short life-cycle sugar beet suitable for molecular genetic analysis. Findings: Five sugar beet lines including NK-219mm-O were crossed with each other and subjected to in vitro culture to form callus. F1s with a NK-219mm-O background generally formed callus efficiently compared to the others, indicating that efficient callus formation is heritable. The regeneration potential was examined based on the phenotypes of calli after placement on regeneration medium. Five phenotypes were observed, of which two phenotypes regenerated shoots or somatic embryo-like structures. Vascular differentiation was evident in regenerable calli, whereas non-regenerable calli lacked normally developed vascular tissues. In a half-diallel cross, the callus-formation efficiency and the regeneration potential of reciprocal F1s progeny having a NK-219mm-O background were high. Finally, we crossed NK-219mm-O with an annual line that had a poor in vitro performance. The callus-formation efficiency and the regeneration potential of reciprocal F1 were high. The regenerated plants showed an annual habitat. Conclusions: Efficient callus formation and the high plant regeneration potential of NK-219mm-O were inherited and expressed in the F1. The annual habitat does not impair these high in vitro performances.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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