HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains

Creative Commons License

Files in This Item:
Manuscript.pdf5.85 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/63713

Title: Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains
Authors: Sugimoto, Masayuki Browse this author
Shimizu, Yoichi Browse this author →KAKEN DB
Yoshioka, Takeshi Browse this author
Wakabayashi, Masato Browse this author
Tanaka, Yukari Browse this author
Higashino, Kenichi Browse this author
Numata, Yoshito Browse this author
Sakai, Shota Browse this author
Kihara, Akio Browse this author →KAKEN DB
Igarashi, Yasuyuld Browse this author
Kuge, Yuji Browse this author →KAKEN DB
Keywords: Imaging mass spectrometry
Fourier transform ion cyclotron resonance
Sphingomyelin
Ceramide synthase
Very long-chain fatty acid
Issue Date: Dec-2015
Publisher: Elsevier
Journal Title: Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids
Volume: 1851
Issue: 12
Start Page: 1554
End Page: 1565
Publisher DOI: 10.1016/j.bbalip.2015.09.004
PMID: 26398595
Abstract: Sphingomyelin (SM) is synthesized by SM synthase (SMS) from ceramide (Cer). SM regulates signaling pathways and maintains organ structure. SM comprises a sphingoid base and differing lengths of acyl-chains, but the importance of its various forms and regulatory synthases is not known. It has been reported that Cer synthase (CerS) has restricted substrate specificity, whereas SMS has no specificity for different lengths of acyl-chains. We hypothesized that the distribution of each SM molecular species was regulated by expression of the CerS family. Thus, we compared the distribution of SM species and CerS mRNA expression using molecular imaging. Spatial distribution of each SM molecular species was investigated using ultra-high-resolution imaging mass spectrometry (IMS). IMS revealed that distribution of SM molecular species varied according to the lengths of acyl-chains found in each brain section. Furthermore, a combination study using in situ hybridization and IMS revealed the spatial expression of CerS1 to be associated with the localization of SM (d18:1/18:0) in cell body-rich gray matter, and CerS2 to be associated with SM (d18:1/24:1) in myelin-rich white matter. Our study is the first comparison of spatial distribution between SM molecular species and CerS isoforms, and revealed their distinct association in the brain. These observations were demonstrated by suppression of CerS2 using siRNA in HepG2 cells; that is, siRNA for CerS2 specifically decreased C22 very long-chain fatty acid (VLCFA)- and C24 VLCFA-containing SMs. Thus, histological analyses of SM species by IMS could be a useful approach to consider their molecular function and regulative mechanism.
Rights: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/63713
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 杉本 正志

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University