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Lactoferrin directly suppresses osteoclastogenesis
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| Title: | Lactoferrin directly suppresses osteoclastogenesis |
| Other Titles: | ラクトフェリンは破骨細胞の分化誘導を直接抑制する |
| Authors: | 加藤, 展教 Browse this author |
| Issue Date: | 24-Mar-2016 |
| Publisher: | Hokkaido University |
| Abstract: | Recent research has shown that lactoferrin indirectly suppresses osteoclastogenesis by affecting osteoblasts and periodontal ligament fibroblasts. However, the mechanism by which lactoferrin directly affects osteoclastogenesis has not been reported. Therefore, the aim of this study was to examine the direct effects of lactoferrin on RANKL-induced osteoclast differentiation of murine osteoclastic RAW 264.7 cells. The number of osteoclasts was determined by counting the number of cells positive for TRAP staining. The effect of lactoferrin on the number of osteoclasts was measured, and the effect on the mRNA expression of osteoclast differentiation markers was assayed using real-time PCR. Lactoferrin decreased the number of osteoclasts (≥2 nuclei) and large osteoclasts (≥8 nuclei) in a dose-dependent manner without affecting the viability of RAW 264.7 cells. In addition, lactoferrin only mediated these effects within 48 h of the beginning of culturing the RAW 264.7 cells with RANKL. Lactoferrin also significantly inhibited RANKL-induced mRNA expression of osteoclasticdifferentiation genes such as NFATc1, RANK, DC-STAMP, and OC-STAMP.These findings suggest that lactoferrin directly suppresses osteoclastogenesis within 48 h of the beginning of culturing the RAW 264.7 cells with RANKL. The use of lactoferrin may be a novel and innovative therapy for treating bone diseases. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第12153号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 山崎 裕, 教授 鈴木 邦明, 教授 土門 卓文, 准教授 吉村 善隆 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/63727 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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