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Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution

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Title: Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution
Authors: Onodera, Yasuyuki Browse this author
Arakawa, Takumi Browse this author
Yui-Kurino, Rika Browse this author
Yamamoto, Masayuki P Browse this author
Kitazaki, Kazuyoshi Browse this author
Ebe, Shigehiko Browse this author
Matsunaga, Muneyuki Browse this author
Taguchi, Kazunori Browse this author
Kuroda, Yosuke Browse this author
Yamashita, Shiko Browse this author
Sakai, Tomoyuki Browse this author
Kinoshita, Toshiro Browse this author →KAKEN DB
Mikami, Tetsuo Browse this author →KAKEN DB
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: F1 hybrid
mitochondrial evolution
mitochondrial gene
nuclear-cytoplasmic interaction
plant mitochondria
substoichiometric DNA
Issue Date: Nov-2015
Publisher: Springer
Journal Title: Euphytica
Volume: 206
Issue: 2
Start Page: 365
End Page: 379
Publisher DOI: 10.1007/s10681-015-1484-2
Abstract: I-12CMS(2) and I-12CMS(3) are sugar beet lines with different sources of cytoplasmic male sterility (CMS) derived from wild beets in Turkey and Pakistan, respectively. We established that I-12CMS(2) has a genetically distinct cytoplasm, but its mitochondrial genome is very similar to I-12CMS(3). Male fertility was assessed in F1 hybrids produced with a common pollen parent. Fertility in the F1's carrying the I-12CMS(3) cytoplasm exceeded that of the F1's with the I-12CMS(2) cytoplasm. Organization of the I-12CMS(2) and I-12CMS(3) mitochondrial genomes were compared based on their physical maps. Mitochondrial genomes of the two strains were largely collinear, except for a large deletion in the noncoding region of I-12CMS(2). Because a mitochondrial orf129 in the I-12CMS(3) cytoplasm is associated with a male sterility phenotype and preservation of orf129 was evident in I-12CMS(2), I-12CMS(2) orf129 was investigated in detail. I-12CMS(2) plants contained three to five times more ORF129 protein than did I-12CMS(3) plants. A single nucleotide substitution, present in the putative promoter region of orf129, appeared to be responsible for the differential accumulation of orf129 transcript. A long N-terminal extension of atp6 is a common feature of some beet CMSs and is found in I-12CMS(2), but the amino acid sequence is unique. I-12CMS(3) mitochondria, but not I-12CMS(2) mitochondria, were found to be heteroplasmic. This heteroplasmy is characterized by a substoichiometric DNA molecule(s) that has at least two I-12CMS(2)-type mitochondrial loci, suggesting the possibility that the I-12CMS(2) mitochondrial genome might have evolved from such a substoichiometric DNA molecule in I-12CMS(3) mitochondria.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/63730
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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