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Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens
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| Title: | Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens |
| Authors: | Suzuki, Akihiro Browse this author | | Shimomura, Kei Browse this author | | Hirose, Makoto Browse this author | | Burdet, Nicolas Browse this author | | Takahashi, Yukio Browse this author →KAKEN DB |
| Issue Date: | 13-Oct-2016 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific reports |
| Volume: | 6 |
| Start Page: | 35060 |
| Publisher DOI: | 10.1038/srep35060 |
| Abstract: | The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray ptychography, which combines X-ray ptychography and X-ray in-line holography, to observe weak-phase objects with a phase resolution better than 0.01 rad, a spatial resolution better than 15 nm, and a field of view larger than 5 mu m. We apply this method to the observation of both the outline and magnetosomes of the magnetotactic bacteria MO-1. Observation of thick samples with high resolution is expected to find broad applications in not only biology but also materials science. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63743 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 明大
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