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Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens

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Title: Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens
Authors: Suzuki, Akihiro Browse this author
Shimomura, Kei Browse this author
Hirose, Makoto Browse this author
Burdet, Nicolas Browse this author
Takahashi, Yukio Browse this author →KAKEN DB
Issue Date: 13-Oct-2016
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 6
Start Page: 35060
Publisher DOI: 10.1038/srep35060
Abstract: The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray ptychography, which combines X-ray ptychography and X-ray in-line holography, to observe weak-phase objects with a phase resolution better than 0.01 rad, a spatial resolution better than 15 nm, and a field of view larger than 5 mu m. We apply this method to the observation of both the outline and magnetosomes of the magnetotactic bacteria MO-1. Observation of thick samples with high resolution is expected to find broad applications in not only biology but also materials science.
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 明大

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