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Histological analysis of arteriovenous anastomosis-like vessels established in the corpus luteum of cows during luteolysis

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タイトル: Histological analysis of arteriovenous anastomosis-like vessels established in the corpus luteum of cows during luteolysis
著者: Nio-Kobayashi, Junko 著作を一覧する
Miyazaki, Kaya 著作を一覧する
Hashiba, Kazuhisa 著作を一覧する
Okuda, Kiyoshi 著作を一覧する
Iwanaga, Toshihiko 著作を一覧する
キーワード: Luteolysis
Arteriovenous anastomosis
Myofibroblasts
PGF2α
TGFβ1
発行日: 2016年10月19日
出版者: BioMed Central
誌名: Journal of ovarian research
巻: 9
開始ページ: 67
出版社 DOI: 10.1186/s13048-016-0277-0
抄録: Background: The mechanisms regulating the function and regression of the corpus luteum (CL) have not yet been elucidated in detail. The regressed CL of cows was previously reported to be filled with unusual vessels like arteriovenous anastomosis (AVA); however how these vessels are being established during luteolysis remains unknown. Methods: The bovine CL at different luteal stages and regressing bovine CL induced by prostaglandin F2α (PGF) were histologically analyzed using light and electron microscopic levels. The changes in mRNA expression of genes encoding α-smooth muscle actin (SMA; Acta2) and transforming growth factor β1 (Tgfb1) in luteal tissues were analyzed by quantitative RT-PCR. Results: AVA-like vessels appeared in the regressed CL with a diameter less than 1.5 cm in which no functional luteal cells and macrophages were observed. Epithelioid cells in the AVA-like vessel wall were immunoreactive for SMA, and the lumen of the vessels were narrow. Immunoreaction for SMA was found in the tunica media of typical arteries and arterioles, and pericytes around capillary vessel. Cells with elongated cytoplasmic processes ―resident fibroblasts expressing vimentin― distributed in the CL parenchyma without any association with blood vessels are also immunoreactive for SMA, and accumulated around arteries and arterioles during the lateluteal stage. In the regressed CL, walls of arteries and arterioles consisted of more than two layers of epithelioid cells positive for both SMA and desmin, suggesting that they are myofibroblasts transformed from fibroblasts. The percentage of the area positive for SMA and the mRNA expression of Acta2 were significantly increased in the regressed CL; however, they did not alter when a luteolytic dose of PGF was injected in vivo and collected within 24 h after the injection. On the other hand, Tgfb1, a known regulator for myofibroblast transformation, was significantly increased in PGF-induced regressing CL as well as in the CL during the late-luteal stage. Conclusions: SMA-positive myofibroblasts accumulates around the arteries and arterioles to form AVA-like vessels during luteolysis in cows. PGF indirectly regulates myofibroblast transformation through enhancing the expression of TGFβ1. These peculiar AVA-like vessels may be involved in the regulation of blood flow in the bovine CL during luteolysis.
資料タイプ: article
URI: http://hdl.handle.net/2115/63825
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 小林 純子

 

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