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Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms
| Title: | Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms |
| Authors: | Ono, Daisuke Browse this author →KAKEN DB | | Honma, Sato Browse this author →KAKEN DB | | Honma, Ken-ichi Browse this author →KAKEN DB |
| Keywords: | behaviour | | bioluminescence | | in vivo recording | | optical fibre | | vasoactive intestinal peptide |
| Issue Date: | Dec-2015 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | European journal of neuroscience |
| Volume: | 42 |
| Issue: | 12 |
| Start Page: | 3128 |
| End Page: | 3137 |
| Publisher DOI: | 10.1111/ejn.13111 |
| PMID: | 26489367 |
| Abstract: | The temporal order of physiology and behaviour in mammals is regulated by the coordination of the master circadian clock in the suprachiasmatic nucleus (SCN) and peripheral clocks in various tissues outside the SCN. Because the circadian oscillator(s) in the olfactory bulb (OB) is regarded as SCN independent, we examined the relationship between the SCN master clock and the circadian clock in the OB. We also examined the role of vasoactive intestinal peptide receptor 2 in the circadian organization of the OB. We continuously monitored the circadian rhythms of a clock gene product PER2 in the SCN and OB of freely moving mice by means of a bioluminescence reporter and an optical fibre implanted in the brain. Robust circadian rhythms were detected in the OB and SCN for up to 19 days. Bilateral SCN lesions abolished the circadian behaviour rhythms and disorganized the PER2 rhythms in the OB. The PER2 rhythms in the OB showed more than one oscillatory component of a similar circadian period, suggesting internal desynchronization of constituent oscillators. By contrast, significant circadian PER2 rhythms were detected in the vasoactive intestinal peptide receptor 2-deficient mice, despite the substantial deterioration or abolition of circadian behavioural rhythms. These findings indicate that the circadian clock in the OB of freely moving mice depends on the SCN master clock but not on the circadian behavioural rhythms. The circadian PER2::LUC rhythm in the cultured OB was as robust as that in the cultured SCN but reset by slice preparation, suggesting that culturing of the slice reinforces the circadian rhythm. |
| Rights: | This is the peer reviewed version of the following article: Ono, D., Honma, S., Honma, K.-i. (2015), Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms. European Journal of Neuroscience, 42: 3128–3137., which has been published in final form at http://doi.org/10.1111/ejn.13111. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/63844 |
| Appears in Collections: | 脳科学研究教育センター (Research and Education Center for Brain Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 本間 さと
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