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Rapid immunocytochemistry based on alternating current electric field using squash smear preparation of central nervous system tumors

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Title: Rapid immunocytochemistry based on alternating current electric field using squash smear preparation of central nervous system tumors
Authors: Moriya, Jun Browse this author
Tanino, Mishie Ann Browse this author →KAKEN DB
Takenami, Tomoko Browse this author
Endoh, Tomoko Browse this author
Urushido, Masana Browse this author
Kato, Yasutaka Browse this author
Wang, Lei Browse this author
Kimura, Taichi Browse this author →KAKEN DB
Tsuda, Masumi Browse this author →KAKEN DB
Nishihara, Hiroshi Browse this author →KAKEN DB
Tanaka, Shinya Browse this author →KAKEN DB
R-IHC Study Group Browse this author
Keywords: Central nervous system (CNS) tumor
Intraoperative diagnosis
Squash smear
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Issue Date: Jan-2016
Publisher: Springer Japan
Journal Title: Brain tumor pathology
Volume: 33
Issue: 1
Start Page: 13
End Page: 18
Publisher DOI: 10.1007/s10014-015-0238-0
PMID: 26546480
Abstract: The role of intraoperative pathological diagnosis for central nervous system (CNS) tumors is crucial for neurosurgery when determining the surgical procedure. Especially, treatment of carmustine (BCNU) wafers requires a conclusive diagnosis of high-grade glioma proven by intraoperative diagnosis. Recently, we demonstrated the usefulness of rapid immunohistochemistry (R-IHC) that facilitates antigen-antibody reaction under alternative current (AC) electric field in the intraoperative diagnosis of CNS tumors; however, a higher proportion of water and lipid in the brain parenchyma sometimes leads to freezing artifacts, resulting in poor quality of frozen sections. On the other hand, squash smear preparation of CNS tumors for cytology does not affect the frozen artifacts, and the importance of smear preparation is now being re-recognized as being better than that of the tissue sections. In this study, we established the rapid immunocytochemistry (R-ICC) protocol for squash smears of CNS tumors using AC electric field that takes only 22 min, and demonstrated its usefulness for semi-quantitative Ki-67/MIB-1 labeling index and CD 20 by R-ICC for intraoperative diagnosis. R-ICC by AC electric field may become a substantial tool for compensating R-IHC and will be applied for broad antibodies in the future.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷野 美智枝

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