Title: | Rapid immunocytochemistry based on alternating current electric field using squash smear preparation of central nervous system tumors |
Authors: | Moriya, Jun Browse this author |
Tanino, Mishie Ann Browse this author →KAKEN DB |
Takenami, Tomoko Browse this author |
Endoh, Tomoko Browse this author |
Urushido, Masana Browse this author |
Kato, Yasutaka Browse this author |
Wang, Lei Browse this author |
Kimura, Taichi Browse this author →KAKEN DB |
Tsuda, Masumi Browse this author →KAKEN DB |
Nishihara, Hiroshi Browse this author →KAKEN DB |
Tanaka, Shinya Browse this author →KAKEN DB |
R-IHC Study Group Browse this author |
Keywords: | Central nervous system (CNS) tumor |
Intraoperative diagnosis |
Squash smear |
Immunocytochemistry (ICC) |
Immunohistochemistry (IHC) |
Issue Date: | Jan-2016 |
Publisher: | Springer Japan |
Journal Title: | Brain tumor pathology |
Volume: | 33 |
Issue: | 1 |
Start Page: | 13 |
End Page: | 18 |
Publisher DOI: | 10.1007/s10014-015-0238-0 |
PMID: | 26546480 |
Abstract: | The role of intraoperative pathological diagnosis for central nervous system (CNS) tumors is crucial for neurosurgery when determining the surgical procedure. Especially, treatment of carmustine (BCNU) wafers requires a conclusive diagnosis of high-grade glioma proven by intraoperative diagnosis. Recently, we demonstrated the usefulness of rapid immunohistochemistry (R-IHC) that facilitates antigen-antibody reaction under alternative current (AC) electric field in the intraoperative diagnosis of CNS tumors; however, a higher proportion of water and lipid in the brain parenchyma sometimes leads to freezing artifacts, resulting in poor quality of frozen sections. On the other hand, squash smear preparation of CNS tumors for cytology does not affect the frozen artifacts, and the importance of smear preparation is now being re-recognized as being better than that of the tissue sections. In this study, we established the rapid immunocytochemistry (R-ICC) protocol for squash smears of CNS tumors using AC electric field that takes only 22 min, and demonstrated its usefulness for semi-quantitative Ki-67/MIB-1 labeling index and CD 20 by R-ICC for intraoperative diagnosis. R-ICC by AC electric field may become a substantial tool for compensating R-IHC and will be applied for broad antibodies in the future. |
Rights: | The final publication is available at link.springer.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/63976 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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