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Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by beta-carotene and Ascorbic Acid in Human HepG2 Cells

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J. Food Sci. v.81(1)p.T275-T281.pdf349.24 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/63978

タイトル: Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by beta-carotene and Ascorbic Acid in Human HepG2 Cells
著者: Darwish, Wageh S. 著作を一覧する
Ikenaka, Yoshinori 著作を一覧する
Nakayama, Shouta M. M. 著作を一覧する
Mizukawa, Hazuki 著作を一覧する
Ishizuka, Mayumi 著作を一覧する
キーワード: AhR
ascorbic acid
beta-carotene
lead
xenobiotic metabolism
発行日: 2016年 1月
出版者: Wiley-Blackwell
誌名: Journal of Food Science
巻: 81
号: 1
開始ページ: T275
終了ページ: T281
出版社 DOI: 10.1111/1750-3841.13162
抄録: Lead (Pb) is an environmental pollutant that can get entry into human body through contaminated foods, drinks, and inhaled air leading to severe biological consequences, and has been responsible for many deaths worldwide. The objectives of this study were 1st to investigate the modulatory effects of environmentally relevant concentrations of Pb on AhR gene battery, which is controlling xenobiotics metabolism. 2nd, trials to reduce Pb-induced adverse effects were done using some phytochemicals like beta-carotene or ascorbic acid. Human hepatoma (HepG2) cell lines were exposed to a wide range of Pb concentrations varying from physiological to toxic levels (0 to 10 mg/L) for 24 h. High Pb concentrations (1 to 10 mg/L) significantly reduced phase I (CYP1A1 and 1A2) and phase II (UGT1A6 and NQO1) xenobiotic metabolizing enzyme mRNA expression in a mechanistic manner through the AhR regulation pathway. Additionally, these Pb concentrations induced oxidative stress in HepG2 cells in terms of production of reactive oxygen species (ROS) and induced heme oxygenase-1 mRNA expression in a concentration-dependent phenomenon. Coexposure of HepG2 cells to physiological concentrations of some micronutrients, like beta-carotene (10 mu M) or ascorbic acid (0.1 mM), along with Pb (1 mg/L) for 24 h significantly reduced the levels of ROS production and recovered AhR mRNA expression into the normal levels. Thus, consumption of foods rich in these micronutrients may help to reduce the adverse effects of lead in areas with high levels of pollution.
Rights: This is the peer reviewed version of the following article: Darwish WS, Ikenaka Y, Nakayama SMM, Mizukawa H, Ishizuka M. 2016. Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β-carotene and Ascorbic Acid in Human HepG2 Cells. J Food Sci 81:T275-81, which has been published in final form at http://dx.doi.org/10.1111/1750-3841.13162. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/63978
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 石塚 真由美

 

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