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A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool

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Title: A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool
Authors: Nzelu, Chukwunonso O. Browse this author
Caceres, Abraham G. Browse this author
Guerrero-Quincho, Silvia Browse this author
Tineo-Villafuerte, Edwin Browse this author
Rodriquez-Delfin, Luis Browse this author
Mimori, Tatsuyuki Browse this author →KAKEN DB
Uezato, Hiroshi Browse this author →KAKEN DB
Katakura, Ken Browse this author →KAKEN DB
Gomez, Eduardo A. Browse this author
Guevara, Angel G. Browse this author
Hashiguchi, Yoshihisa Browse this author →KAKEN DB
Kato, Hirotomo Browse this author →KAKEN DB
Keywords: Leishmania
LAMP
FTA
Malachite green
Peru
Issue Date: Jan-2016
Publisher: Elsevier
Journal Title: Acta Tropica
Volume: 153
Start Page: 116
End Page: 119
Publisher DOI: 10.1016/j.actatropica.2015.10.013
PMID: 26516109
Abstract: Leishmaniasis remains one of the world's most neglected diseases, and early detection of the infectious agent, especially in developing countries, will require a simple and rapid test. In this study, we established a quick, one-step, single-tube, highly sensitive loop-mediated isothermal amplification (LAMP) assay for rapid detection of Leishmania DNA from tissue materials spotted on an FTA card. An FTA-LAMP with pre-added malachite green was performed at 64 degrees C for 60 mm using a heating block and/or water bath and DNA amplification was detected immediately after incubation. The LAMP assay had high detection sensitivity down to a level of 0.01 parasites per mu l. The field- and clinic-applicability of the colorimetric FTA-LAMP assay was demonstrated with 122 clinical samples collected from patients suspected of having cutaneous leishmaniasis in Peru, from which 71 positives were detected. The LAMP assay in combination with an FTA card described here is rapid and sensitive, as well as simple to perform, and has great potential usefulness for diagnosis and surveillance of leishmaniasis in endemic areas. (C) 2015 Elsevier B.V. All rights reserved.
Rights: ©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/63983
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 大智

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