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Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger

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Biosci. Biotechnol. Biochem.80-9_1747-1752.pdf2.81 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/64479

Title: Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger
Authors: Liao, Julan Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Ishihara, Keigo Browse this author
Yamori, Yoshinori Browse this author
Iki, Shigeo Browse this author
Tagami, Takayoshi Browse this author
Mori, Haruhide Browse this author →KAKEN DB
Chiba, Seiya Browse this author →KAKEN DB
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: glycoside hydrolase family 27
-galactosidase
substrate inhibition
substrate specificity
Issue Date: Sep-2016
Publisher: Taylor & Francis
Journal Title: Bioscience biotechnology and biochemistry
Volume: 80
Issue: 9
Start Page: 1747
End Page: 1752
Publisher DOI: 10.1080/09168451.2015.1136884
PMID: 26856407
Abstract: The recombinant AglB produced by Pichia pastoris exhibited substrate inhibition behavior for the hydrolysis of p-nitrophenyl -galactoside, whereas it hydrolyzed the natural substrates, including galactomanno-oligosaccharides and raffinose family oligosaccharides, according to the Michaelian kinetics. These contrasting kinetic behaviors can be attributed to the difference in the dissociation constant of second substrate from the enzyme and/or to the ability of the leaving group of the substrates. The enzyme displays the grater k(cat)/K-m values for hydrolysis of the branched -galactoside in galactomanno-oligosaccharides than that of raffinose and stachyose. A sequence comparison suggested that AglB had a shallow active-site pocket, and it can allow to hydrolyze the branched -galactosides, but not linear raffinose family oligosaccharides.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 09 Feb 2016, available online: http://wwww.tandfonline.com/10.1080/09168451.2015.1136884.
Type: article (author version)
URI: http://hdl.handle.net/2115/64479
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

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