|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger
| Title: | Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger |
| Authors: | Liao, Julan Browse this author | | Okuyama, Masayuki Browse this author →KAKEN DB | | Ishihara, Keigo Browse this author | | Yamori, Yoshinori Browse this author | | Iki, Shigeo Browse this author | | Tagami, Takayoshi Browse this author | | Mori, Haruhide Browse this author →KAKEN DB | | Chiba, Seiya Browse this author →KAKEN DB | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | glycoside hydrolase family 27 | | α-galactosidase | | substrate inhibition | | substrate specificity |
| Issue Date: | Sep-2016 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience biotechnology and biochemistry |
| Volume: | 80 |
| Issue: | 9 |
| Start Page: | 1747 |
| End Page: | 1752 |
| Publisher DOI: | 10.1080/09168451.2015.1136884 |
| PMID: | 26856407 |
| Abstract: | The recombinant AglB produced by Pichia pastoris exhibited substrate inhibition behavior for the hydrolysis of p-nitrophenyl -galactoside, whereas it hydrolyzed the natural substrates, including galactomanno-oligosaccharides and raffinose family oligosaccharides, according to the Michaelian kinetics. These contrasting kinetic behaviors can be attributed to the difference in the dissociation constant of second substrate from the enzyme and/or to the ability of the leaving group of the substrates. The enzyme displays the grater k(cat)/K-m values for hydrolysis of the branched -galactoside in galactomanno-oligosaccharides than that of raffinose and stachyose. A sequence comparison suggested that AglB had a shallow active-site pocket, and it can allow to hydrolyze the branched -galactosides, but not linear raffinose family oligosaccharides. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 09 Feb 2016, available online: http://wwww.tandfonline.com/10.1080/09168451.2015.1136884. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64479 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 木村 淳夫
|
