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Title: アスパラガス野外茎頂の凍結保存における前処理の影響
Other Titles: Effects of Pretreatments on Cryopreservation in Asparagus Shoot Apices
Authors: 実山, 豊1 Browse this author →KAKEN DB
鈴木, 卓2 Browse this author
原田, 隆3 Browse this author
藤川, 清三4 Browse this author
Authors(alt): JITSUYAMA, Yutaka1
SUZUKI, Takashi2
HARADA, Takashi3
Keywords: 茎頂
Shoot apices
Freezing tolerance
Ultrastructural changes
Issue Date: 28-Dec-1995
Publisher: 低温生物工学会
Journal Title: 低温生物工学会誌
Volume: 41
Issue: 2
Start Page: 50
End Page: 57
Abstract: Shoot apices of asparagus (Asparagus officinalis L.) can be successfully cryopreserved (by freezing at a rate of 0.5℃/min in the presence of 8% DMSO and 0.2 M sorbitol) after pretreat­ ments either by preculture in media containing 0.5 M glucose for 2 days or by cold acclimation at O℃ for 20 days. In the present study, we examined the effects of pretreatments for subsequent successful cryopreservation of the shoot apices. Freezing tolerance, as revealed by LT50 under equilibrium-freezing, was 20℃ in both specimens with pretreatment by 0.5 M glucose and by cold acclimation, while LT50 was -7℃ in control specimens. Both pretreatments produced distinct cellular ultrastructural changes as observed with ultra-thin section electron microscopy. Number of plastids rich in starch granules increased in precultured specimens, whereas those poor in the starch granules increased in cold acclimated specimens. Occurrence of freezing injury by equilibrium-freezing, as observed with a freeze-fracture replica technique, was associated with formation of aparticulate domains with accompanying fracture-jump lesions in the plasma membranes, not only in precultured specimens with 0.5 M glucose and in cold acclimated specimens, but also in control specimens.
Rights: 利用は著作権の範囲内に限られる
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 実山 豊

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