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Adhesion of Osteoblast-like Cells (Saos-2) on Micro-/Submicro-Patterned Apatite Scaffolds Fabricated with Apatite Cement Paste by Micro-Molding

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Title: Adhesion of Osteoblast-like Cells (Saos-2) on Micro-/Submicro-Patterned Apatite Scaffolds Fabricated with Apatite Cement Paste by Micro-Molding
Authors: Akasaka, Tsukasa Browse this author →KAKEN DB
Miyaji, Hirofumi Browse this author →KAKEN DB
Kaga, Naoyuki Browse this author
Yokoyama, Atsuro Browse this author →KAKEN DB
Abe, Shigeaki Browse this author →KAKEN DB
Yoshida, Yasuhiro Browse this author →KAKEN DB
Keywords: patterned apatite
micro-molding
micro/submicro
Saos-2
cell adhesion
Issue Date: Dec-2016
Publisher: ナノバイオメディカル学会
Nano Biomedical Society
Journal Title: Nano Biomedicine
Volume: 8
Issue: 2
Start Page: 112
End Page: 122
Abstract: In the present study, we developed patterned apatites with grooves, pillars, and holes by micro-molding. The effects of the patterns on adhesion of the human osteoblastic cell line Saos-2 were investigated. The patterned apatites were fabricated with apatite cement paste using the mi-cro-molding method. The number of attached cells and ratio of cell spreading were estimated by a cell adhesion assay using Saos-2. The resulting patterns of grooves, holes, and pillars at the mi-cro-/submicro-level were easily transferred using the corresponding mold. Saos-2 cells were well orientated on the grooves and filopodia were radially elongated on pillars. The number of attached cells on the patterned apatite was higher than that on the planar apatite. Interestingly, the tendency of increasing of ratios of spreading cells was similar to that of decreasing of water contact angle on apatite pillars. These results show that the adhesion of Saos-2 cells was affected by the type and size of the apatite patterns.
Type: article
URI: http://hdl.handle.net/2115/64538
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 赤坂 司

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