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Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin

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Title: Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin
Authors: Akasaka, Tsukasa Browse this author →KAKEN DB
Imamura, Takuya Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Kaga, Naoyuki Browse this author
Yokoyama, Atsuro Browse this author →KAKEN DB
Yoshida, Yasuhiro Browse this author
Keywords: Biophysics, medical physics, and biomedical engineering
Surface structure, morphology, roughness, and topography
Biological aspects of nano-structures
Dental composite resin
Human gingival fibroblast
Issue Date: 10-Dec-2016
Publisher: 日本表面科学会
The Surface Science Society of Japan
Journal Title: e-Journal of Surface Science and Nanotechnology
Volume: 14
Start Page: 225
End Page: 230
Publisher DOI: 10.1380/ejssnt.2016.225
Abstract: Groove patterns on the surface of implants act as an effective barrier to the apical migration of epithelial attachment, after which the grooves facilitate gingival fibroblast attachment. Cell alignment on grooves is largely influenced by the adsorbed protein type. However, cell attachment and cell alignment properties of micro/nano-grooved dental composite resins using osteoblasts and fibroblasts have not been investigated. Further, the effect of saliva-related protein adsorption has not investigated. In this study, we prepared composite resins with grooves that were 2 μm, 1 μm, and 500 nm wide and estimated the effect of pre-coating of some proteins, mainly mucin, on attachment and alignment of human gingival fibroblasts(HGF). In the cell attachment assay on mucin-coated grooves, the number of attached cells on mucin-coated planar or grooved composite resins was lower compared to that on both composite resins without pre-coating of mucin. Interestingly, the number of attached cells on grooves pre-coated with mucin was 5.7-fold higher than those on planar pre-coated with mucin. Grooves at the micro/nano level may act as a hook for floating cells during the cell attachment assay. Furthermore, the degree of cell alignment was strongly dependent on the pre-coating protein types. The cells were radially spread or round-shaped, but not have sufficient alignment on non-, mucin-, and albumin-coated grooves. Although the cells were attached on the grooves, they were not aligned along the direction of grooves. The cells on fetal bovine serum- or fibronectin-coated grooves exhibited good alignment in the groove direction, particularly on fibronectin-coated grooves. Thus, our patterning method creates an effective seal between soft tissue and dental materials to protect against microorganism invasion.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 赤坂 司

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