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Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin

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タイトル: Effect of Protein Adsorption on Alignment of Human Gingival Fibroblasts on Grooved Composite Resin
著者: Akasaka, Tsukasa 著作を一覧する
Imamura, Takuya 著作を一覧する
Miyaji, Hirofumi 著作を一覧する
Kaga, Naoyuki 著作を一覧する
Yokoyama, Atsuro 著作を一覧する
Yoshida, Yasuhiro 著作を一覧する
キーワード: Biophysics, medical physics, and biomedical engineering
Surface structure, morphology, roughness, and topography
Biological aspects of nano-structures
Dental composite resin
Human gingival fibroblast
発行日: 2016年12月10日
出版者: 日本表面科学会
The Surface Science Society of Japan
誌名: e-Journal of Surface Science and Nanotechnology
巻: 14
開始ページ: 225
終了ページ: 230
出版社 DOI: 10.1380/ejssnt.2016.225
抄録: Groove patterns on the surface of implants act as an effective barrier to the apical migration of epithelial attachment, after which the grooves facilitate gingival fibroblast attachment. Cell alignment on grooves is largely influenced by the adsorbed protein type. However, cell attachment and cell alignment properties of micro/nano-grooved dental composite resins using osteoblasts and fibroblasts have not been investigated. Further, the effect of saliva-related protein adsorption has not investigated. In this study, we prepared composite resins with grooves that were 2 μm, 1 μm, and 500 nm wide and estimated the effect of pre-coating of some proteins, mainly mucin, on attachment and alignment of human gingival fibroblasts(HGF). In the cell attachment assay on mucin-coated grooves, the number of attached cells on mucin-coated planar or grooved composite resins was lower compared to that on both composite resins without pre-coating of mucin. Interestingly, the number of attached cells on grooves pre-coated with mucin was 5.7-fold higher than those on planar pre-coated with mucin. Grooves at the micro/nano level may act as a hook for floating cells during the cell attachment assay. Furthermore, the degree of cell alignment was strongly dependent on the pre-coating protein types. The cells were radially spread or round-shaped, but not have sufficient alignment on non-, mucin-, and albumin-coated grooves. Although the cells were attached on the grooves, they were not aligned along the direction of grooves. The cells on fetal bovine serum- or fibronectin-coated grooves exhibited good alignment in the groove direction, particularly on fibronectin-coated grooves. Thus, our patterning method creates an effective seal between soft tissue and dental materials to protect against microorganism invasion.
資料タイプ: article
URI: http://hdl.handle.net/2115/64545
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 赤坂 司

 

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