| Title: | Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs |
| Authors: | Ichii, Osamu Browse this author →KAKEN DB |
| Ohta, Hiroshi Browse this author →KAKEN DB |
| Horino, Taro Browse this author |
| Nakamura, Teppei Browse this author |
| Hosotani, Marina Browse this author |
| Mizoguchi, Tatsuya Browse this author |
| Morishita, Keitaro Browse this author →KAKEN DB |
| Nakamura, Kensuke Browse this author |
| Hoshino, Yuki Browse this author →KAKEN DB |
| Takagi, Satoshi Browse this author →KAKEN DB |
| Sasaki, Noboru Browse this author |
| Takiguchi, Mitsuyoshi Browse this author →KAKEN DB |
| Sato, Ryo Browse this author |
| Oyamada, Kazuhisa Browse this author |
| Kon, Yasuhiro Browse this author →KAKEN DB |
| Issue Date: | 11-Jan-2017 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific Reports |
| Volume: | 7 |
| Start Page: | 40340 |
| Publisher DOI: | 10.1038/srep40340 |
| Abstract: | MicroRNAs act as post-transcriptional regulators, and urinary exosome (UExo)-derived microRNAs may be used as biomarkers. Herein, we screened for UExo-derived microRNAs reflecting kidney disease (KD) status in dogs. Examined dogs were divided into healthy kidney control (HC) and KD groups according to renal dysfunction. We confirmed the appearance of UExo having irregular globe-shapes in a dog by immunoblot detection of the exosome markers, TSG101 and CD9. Based on our previous data using KD model mice and the data obtained herein by next generation sequencing of UExo-derived microRNAs in dogs, miR-26a, miR-146a, miR-486, miR-21a, and miR-10a/b were selected as candidate microRNAs. In particular, UExo-derived miR-26a and miR-10a/b were significantly decreased in KD dogs, and miR-26a levels negatively correlated with deteriorated renal function compared to the other miRNAs. UExo-derived miR-21a levels corrected or not to that of internal control microRNAs in UExo, miR-26a and miR-191, significantly increased with renal dysfunction. In kidney tissues, the decrease of miR-26a and miR-10a/ b in the glomerulus and miR-10b in the tubulointerstitium negatively correlated with deteriorated renal function and histopathology. Increased miR-21a in the tubulointerstitium rather than in the glomerulus correlated with deteriorated renal histopathology. In conclusion, microRNAs reflecting the changes in renal function and histopathology in dogs were identified in this study. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/64596 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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