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Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs


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タイトル: Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs
著者: Ichii, Osamu 著作を一覧する
Ohta, Hiroshi 著作を一覧する
Horino, Taro 著作を一覧する
Nakamura, Teppei 著作を一覧する
Hosotani, Marina 著作を一覧する
Mizoguchi, Tatsuya 著作を一覧する
Morishita, Keitaro 著作を一覧する
Nakamura, Kensuke 著作を一覧する
Hoshino, Yuki 著作を一覧する
Takagi, Satoshi 著作を一覧する
Sasaki, Noboru 著作を一覧する
Takiguchi, Mitsuyoshi 著作を一覧する
Sato, Ryo 著作を一覧する
Oyamada, Kazuhisa 著作を一覧する
Kon, Yasuhiro 著作を一覧する
発行日: 2017年 1月11日
出版者: Nature Publishing Group
誌名: Scientific Reports
巻: 7
開始ページ: 40340
出版社 DOI: 10.1038/srep40340
抄録: MicroRNAs act as post-transcriptional regulators, and urinary exosome (UExo)-derived microRNAs may be used as biomarkers. Herein, we screened for UExo-derived microRNAs reflecting kidney disease (KD) status in dogs. Examined dogs were divided into healthy kidney control (HC) and KD groups according to renal dysfunction. We confirmed the appearance of UExo having irregular globe-shapes in a dog by immunoblot detection of the exosome markers, TSG101 and CD9. Based on our previous data using KD model mice and the data obtained herein by next generation sequencing of UExo-derived microRNAs in dogs, miR-26a, miR-146a, miR-486, miR-21a, and miR-10a/b were selected as candidate microRNAs. In particular, UExo-derived miR-26a and miR-10a/b were significantly decreased in KD dogs, and miR-26a levels negatively correlated with deteriorated renal function compared to the other miRNAs. UExo-derived miR-21a levels corrected or not to that of internal control microRNAs in UExo, miR-26a and miR-191, significantly increased with renal dysfunction. In kidney tissues, the decrease of miR-26a and miR-10a/ b in the glomerulus and miR-10b in the tubulointerstitium negatively correlated with deteriorated renal function and histopathology. Increased miR-21a in the tubulointerstitium rather than in the glomerulus correlated with deteriorated renal histopathology. In conclusion, microRNAs reflecting the changes in renal function and histopathology in dogs were identified in this study.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 市居 修


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