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Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/64596

Title: Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs
Authors: Ichii, Osamu Browse this author →KAKEN DB
Ohta, Hiroshi Browse this author →KAKEN DB
Horino, Taro Browse this author
Nakamura, Teppei Browse this author
Hosotani, Marina Browse this author
Mizoguchi, Tatsuya Browse this author
Morishita, Keitaro Browse this author →KAKEN DB
Nakamura, Kensuke Browse this author
Hoshino, Yuki Browse this author →KAKEN DB
Takagi, Satoshi Browse this author →KAKEN DB
Sasaki, Noboru Browse this author
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Sato, Ryo Browse this author
Oyamada, Kazuhisa Browse this author
Kon, Yasuhiro Browse this author →KAKEN DB
Issue Date: 11-Jan-2017
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 7
Start Page: 40340
Publisher DOI: 10.1038/srep40340
Abstract: MicroRNAs act as post-transcriptional regulators, and urinary exosome (UExo)-derived microRNAs may be used as biomarkers. Herein, we screened for UExo-derived microRNAs reflecting kidney disease (KD) status in dogs. Examined dogs were divided into healthy kidney control (HC) and KD groups according to renal dysfunction. We confirmed the appearance of UExo having irregular globe-shapes in a dog by immunoblot detection of the exosome markers, TSG101 and CD9. Based on our previous data using KD model mice and the data obtained herein by next generation sequencing of UExo-derived microRNAs in dogs, miR-26a, miR-146a, miR-486, miR-21a, and miR-10a/b were selected as candidate microRNAs. In particular, UExo-derived miR-26a and miR-10a/b were significantly decreased in KD dogs, and miR-26a levels negatively correlated with deteriorated renal function compared to the other miRNAs. UExo-derived miR-21a levels corrected or not to that of internal control microRNAs in UExo, miR-26a and miR-191, significantly increased with renal dysfunction. In kidney tissues, the decrease of miR-26a and miR-10a/ b in the glomerulus and miR-10b in the tubulointerstitium negatively correlated with deteriorated renal function and histopathology. Increased miR-21a in the tubulointerstitium rather than in the glomerulus correlated with deteriorated renal histopathology. In conclusion, microRNAs reflecting the changes in renal function and histopathology in dogs were identified in this study.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/64596
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 市居 修

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