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Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation

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Title: Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation
Authors: Goto, Keiko Browse this author
Kimura, Taichi Browse this author →KAKEN DB
Kitamura, Nobuto Browse this author →KAKEN DB
Semba, Shingo Browse this author →KAKEN DB
Ohmiya, Yoshihiro Browse this author →KAKEN DB
Aburatani, Sachiyo Browse this author →KAKEN DB
Matsukura, Satoko Browse this author
Tsuda, Masumi Browse this author →KAKEN DB
Kurokawa, Takayuki Browse this author →KAKEN DB
Gong, Jian Ping Browse this author →KAKEN DB
Tanaka, Shinya Browse this author →KAKEN DB
Yasuda, Kazunori Browse this author →KAKEN DB
Keywords: PAMPS hydrogel
chondrogenic differentiation
signaling pathway
bone morphogenetic protein
transforming growth factor
BMP
TGF
Issue Date: Mar-2016
Publisher: Wiley-Blackwell
Journal Title: Journal of biomedical materials research part A
Volume: 104
Issue: 3
Start Page: 734
End Page: 746
Publisher DOI: 10.1002/jbm.a.35615
PMID: 26566602
Abstract: The purposes of this study were to identify signaling pathways that were specifically activated in ATDC5 cells cultured on poly (2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) gel in insulin-free maintenance medium and to evaluate the significance of the determined signaling pathways in the chondrogenic differentiation induced by this gel. In this study, ATDC5 cells cultured on PAMPS gel using the maintenance medium without insulin (PAMPS Culture) were compared with cells cultured on polystyrene using the differentiation medium containing insulin (PS-I Culture). The microarray analysis, Western blot analysis, and real-time PCR analysis demonstrated that the TGF-β/BMP signaling pathway was significantly enhanced at Days 1, 2, and 3 in the PAMPS Culture when compared with the PS-I Culture. Inhibition of the BMP type-I receptor reduced the phosphorylation level of Smad1/5 and expression of type-2 collagen and aggrecan mRNA in the cells accompanied by a reduction in cell aggregation at Day 13 in the PAMPS Culture. The inhibition of the TGF-β/BMP signaling pathway significantly inhibited the chondrogenic differentiation induced by the PAMPS gel. The present study demonstrated that synthetic PAMPS gel activates the TGF-β/BMP/Smad signaling pathway in the ATDC5 cells in the absence of insulin, and that this activation plays a significant role in the chondrogenic differentiation induced by PAMPS gel.
Rights: This is the peer reviewed version of the following article: Goto K, Kimura T, Kitamura N, Semba S, Ohmiya Y, Aburatani S, Matsukura S, Tsuda M, Kurokawa T, Gong JP, Tanaka S, Yasuda K. 2016. Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation. J Biomed Mater Res Part A 2016:104A:734–746., which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35615. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/64638
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 北村 信人

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