| Title: | Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation |
| Authors: | Goto, Keiko Browse this author |
| Kimura, Taichi Browse this author →KAKEN DB |
| Kitamura, Nobuto Browse this author →KAKEN DB |
| Semba, Shingo Browse this author →KAKEN DB |
| Ohmiya, Yoshihiro Browse this author →KAKEN DB |
| Aburatani, Sachiyo Browse this author →KAKEN DB |
| Matsukura, Satoko Browse this author |
| Tsuda, Masumi Browse this author →KAKEN DB |
| Kurokawa, Takayuki Browse this author →KAKEN DB |
| Gong, Jian Ping Browse this author →KAKEN DB |
| Tanaka, Shinya Browse this author →KAKEN DB |
| Yasuda, Kazunori Browse this author →KAKEN DB |
| Keywords: | PAMPS hydrogel |
| chondrogenic differentiation |
| signaling pathway |
| bone morphogenetic protein |
| transforming growth factor |
| BMP |
| TGF |
| Issue Date: | Mar-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Journal of biomedical materials research part A |
| Volume: | 104 |
| Issue: | 3 |
| Start Page: | 734 |
| End Page: | 746 |
| Publisher DOI: | 10.1002/jbm.a.35615 |
| PMID: | 26566602 |
| Abstract: | The purposes of this study were to identify signaling pathways that were specifically activated in ATDC5 cells cultured on poly (2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) gel in insulin-free maintenance medium and to evaluate the significance of the determined signaling pathways in the chondrogenic differentiation induced by this gel. In this study, ATDC5 cells cultured on PAMPS gel using the maintenance medium without insulin (PAMPS Culture) were compared with cells cultured on polystyrene using the differentiation medium containing insulin (PS-I Culture). The microarray analysis, Western blot analysis, and real-time PCR analysis demonstrated that the TGF-β/BMP signaling pathway was significantly enhanced at Days 1, 2, and 3 in the PAMPS Culture when compared with the PS-I Culture. Inhibition of the BMP type-I receptor reduced the phosphorylation level of Smad1/5 and expression of type-2 collagen and aggrecan mRNA in the cells accompanied by a reduction in cell aggregation at Day 13 in the PAMPS Culture. The inhibition of the TGF-β/BMP signaling pathway significantly inhibited the chondrogenic differentiation induced by the PAMPS gel. The present study demonstrated that synthetic PAMPS gel activates the TGF-β/BMP/Smad signaling pathway in the ATDC5 cells in the absence of insulin, and that this activation plays a significant role in the chondrogenic differentiation induced by PAMPS gel. |
| Rights: | This is the peer reviewed version of the following article: Goto K, Kimura T, Kitamura N, Semba S, Ohmiya Y, Aburatani S, Matsukura S, Tsuda M, Kurokawa T, Gong JP, Tanaka S, Yasuda K. 2016. Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation. J Biomed Mater Res Part A 2016:104A:734–746., which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35615. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64638 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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